PMID- 19049328
OWN - NLM
STAT- MEDLINE
DCOM- 20090716
LR  - 20151119
IS  - 1523-4681 (Electronic)
IS  - 0884-0431 (Linking)
VI  - 24
IP  - 4
DP  - 2009 Apr
TI  - Experimental folate and vitamin B12 deficiency does not alter bone quality in 
      rats.
PG  - 589-96
LID - 10.1359/jbmr.081211 [doi]
AB  - Hyperhomocysteinemia (HHCY) has been linked to fragility fractures and 
      osteoporosis. Folate and vitamin B(12) deficiencies are among the main causes of 
      HHCY. However, the impact of these vitamins on bone health has been poorly 
      studied. This study analyzed the effect of folate and vitamin B(12) deficiency on 
      bone in rats. We used two groups of rats: a control group (Co, n = 10) and a 
      vitamin-deficient group (VitDef, n = 10). VitDef animals were fed for 12 wk with 
      a folate- and vitamin B(12)-free diet. Co animals received an equicaloric control 
      diet. Tissue and plasma concentrations of homocysteine (HCY), 
      S-adenosyl-homocysteine (SAH), and S-adenosyl-methionine (SAM) were measured. 
      Bone quality was assessed by biomechanical testing (maximum force of an axial 
      compression test; F(max)), histomorphometry (bone area/total area; B.Ar./T.Ar.], 
      and the measurement of biochemical bone turnover markers (osteocalcin, collagen I 
      C-terminal cross-laps [CTX]). VitDef animals developed significant HHCY (Co 
      versus VitDef: 6.8 +/- 2.7 versus 61.1 +/- 12.8 microM, p < 0.001) that was 
      accompanied by a high plasma concentration of SAH (Co versus VitDef: 24.1 +/- 5.9 
      versus 86.4 +/- 44.3 nM, p < 0.001). However, bone tissue concentrations of HCY, 
      SAH, and SAM were similar in the two groups. Fmax, B.Ar./T.Ar., OC, and CTX did 
      not differ between VitDef and Co animals, indicating that bone quality was not 
      affected. Folate and vitamin B(12) deficiency induces distinct HHCY but has no 
      effect on bone health in otherwise healthy adult rats. The unchanged HCY 
      metabolism in bone is the most probable explanation for the missing effect of the 
      vitamin-free diet on bone.
FAU - Herrmann, Markus
AU  - Herrmann M
AD  - Department of Clinical Chemistry and Laboratory Medicine, University Hospital of 
      Saarland, Homburg, Germany. markusherr@aol.com
FAU - Wildemann, Britt
AU  - Wildemann B
FAU - Wagner, Alexandra
AU  - Wagner A
FAU - Wolny, Martin
AU  - Wolny M
FAU - Schorr, Heike
AU  - Schorr H
FAU - Taban-Shomal, Omid
AU  - Taban-Shomal O
FAU - Umanskaya, Natalia
AU  - Umanskaya N
FAU - Ross, Steffen
AU  - Ross S
FAU - Garcia, Patric
AU  - Garcia P
FAU - Hubner, Ulrich
AU  - Hubner U
FAU - Herrmann, Wolfgang
AU  - Herrmann W
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Bone Miner Res
JT  - Journal of bone and mineral research : the official journal of the American 
      Society for Bone and Mineral Research
JID - 8610640
RN  - 0 (Biomarkers)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - 7LP2MPO46S (S-Adenosylmethionine)
RN  - 979-92-0 (S-Adenosylhomocysteine)
SB  - IM
MH  - Animals
MH  - Biomarkers/blood
MH  - Biomechanical Phenomena
MH  - Body Weight
MH  - Bone Remodeling
MH  - Bone and Bones/*physiopathology
MH  - Disease Models, Animal
MH  - Female
MH  - Folic Acid Deficiency/blood/chemically induced/*complications/*physiopathology
MH  - Homocysteine/blood/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - S-Adenosylhomocysteine/blood
MH  - S-Adenosylmethionine/blood
MH  - Vitamin B 12 Deficiency/blood/chemically induced/*complications/*physiopathology
EDAT- 2008/12/04 09:00
MHDA- 2009/07/17 09:00
CRDT- 2008/12/04 09:00
PHST- 2008/12/04 09:00 [pubmed]
PHST- 2009/07/17 09:00 [medline]
PHST- 2008/12/04 09:00 [entrez]
AID - 10.1359/jbmr.081211 [doi]
PST - ppublish
SO  - J Bone Miner Res. 2009 Apr;24(4):589-96. doi: 10.1359/jbmr.081211.