PMID- 19049879
OWN - NLM
STAT- MEDLINE
DCOM- 20090304
LR  - 20090216
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 26
IP  - 2
DP  - 2009 Feb
TI  - Molecular cloning and expression of MyD88 in large yellow croaker, Pseudosciaena 
      crocea.
PG  - 249-55
LID - 10.1016/j.fsi.2008.10.014 [doi]
AB  - Myeloid differentiation factor 88 (MyD88) is an adaptor protein involved in the 
      interleukin-1 receptor and Toll-like receptor-induced activation of nuclear 
      factor-kappaB (NF-kappaB). In this report, the full-length cDNA of MyD88 was 
      cloned from the large yellow croaker, Pseudosciaena crocea. It was of 1574 bp, 
      including a 5'-terminal untranslated region (UTR) of 89 bp, a 3'-terminal UTR of 
      621bp and an open reading frame (ORF) of 864 bp encoding a polypeptide of 287 
      amino acids. It contained a typical death domain at the N-terminal and a 
      conservative Toll/IL-1R (TIR) domain structure at the C-terminal. The 
      quantitative real-time reverse transcription PCR analysis revealed a broad 
      expression of MyD88 with the highest expression in the spleen and the weakest 
      expression in the muscle. The expression of MyD88 after challenge with 
      formalin-inactivated Gram-negative bacterium Vibrio parahaemolyticus was tested 
      in blood, spleen and liver. It suggested that the highest expression was in the 
      spleen (p<0.05) with 1.9 times (at 48 h) as much as that in the control and the 
      lowest expression of MyD88 was in the liver (p<0.05) with 0.29 times (at 3h) of 
      that in the control. These results indicated that as a universal key adaptor in 
      the Toll-like receptor pathway in mammals, MyD88 might play an important role in 
      large yellow croaker defense against pathogenic infection.
FAU - Yao, Cui-Luan
AU  - Yao CL
AD  - Key Laboratory of Science and Technology for Aquaculture and Food Safety of 
      Fujian Province University, Fisheries College/Fisheries Biotechnology Institute, 
      Jimei University, Xiamen 361021, China.
FAU - Kong, Peng
AU  - Kong P
FAU - Wang, Zhi-Yong
AU  - Wang ZY
FAU - Ji, Pei-Feng
AU  - Ji PF
FAU - Liu, Xian-De
AU  - Liu XD
FAU - Cai, Ming-Yi
AU  - Cai MY
FAU - Han, Xue-Zhe
AU  - Han XZ
LA  - eng
SI  - GENBANK/EU978950
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081117
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Myeloid Differentiation Factor 88)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation
MH  - Liver/immunology
MH  - Molecular Sequence Data
MH  - Myeloid Differentiation Factor 88/blood/chemistry/*genetics/*immunology
MH  - Perciformes/classification/*genetics/*immunology
MH  - Phylogeny
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Spleen/immunology
MH  - Vibrio Infections/immunology/veterinary
MH  - Vibrio parahaemolyticus/immunology
EDAT- 2008/12/04 09:00
MHDA- 2009/03/05 09:00
CRDT- 2008/12/04 09:00
PHST- 2008/08/06 00:00 [received]
PHST- 2008/10/19 00:00 [revised]
PHST- 2008/10/27 00:00 [accepted]
PHST- 2008/12/04 09:00 [pubmed]
PHST- 2009/03/05 09:00 [medline]
PHST- 2008/12/04 09:00 [entrez]
AID - S1050-4648(08)00255-6 [pii]
AID - 10.1016/j.fsi.2008.10.014 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2009 Feb;26(2):249-55. doi: 10.1016/j.fsi.2008.10.014. 
      Epub 2008 Nov 17.