PMID- 19052091
OWN - NLM
STAT- MEDLINE
DCOM- 20090213
LR  - 20211020
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 83
IP  - 4
DP  - 2009 Feb
TI  - Differential sensitivity of differentiated epithelial cells to respiratory 
      viruses reveals different viral strategies of host infection.
PG  - 1962-8
LID - 10.1128/JVI.01271-08 [doi]
AB  - To address the initiation of virus infection in the respiratory tract, we 
      established two culture systems for differentiated bovine airway epithelial cells 
      (BAEC). Filter-grown BAEC differentiated under air-liquid interface (ALI) 
      conditions to generate a pseudo-stratified mucociliary epithelium. Alternatively, 
      precision-cut lung slices (PCLS) from the bovine airways were generated that 
      retained the original composition and distribution of differentiated epithelial 
      cells. With both systems, epithelial cells were readily infected by bovine 
      parainfluenza virus 3 (BPIV3). Ciliated cells were the most prominent cell type 
      affected by BPIV3. Surprisingly, differentiated BAEC were resistant to infection 
      by bovine respiratory syncytial virus (BRSV), when the virus was applied at the 
      same multiplicity of infection that was sufficient for infection by BPIV3. In the 
      case of PCLS, infection by BRSV was observed in cells located in lower cell 
      layers but not in epithelial cells facing the lumen of the airways. The identity 
      of the infected cells could not be determined because of a lack of specific 
      antibodies. Increasing the virus titer 30-fold resulted in infection of the ALI 
      cultures of BAEC, whereas in PCLS the ciliated epithelium was still refractory to 
      infection by BRSV. These results indicate that differentiated BAEC are readily 
      infected by BPIV3 but rather resistant to infection by BRSV. Disease caused by 
      BRSV may require that calves encounter environmental stimuli that render BAEC 
      susceptible to infection.
FAU - Goris, Katherina
AU  - Goris K
AD  - Institute of Virology, University of Veterinary Medicine Hannover, Germany.
FAU - Uhlenbruck, Sabine
AU  - Uhlenbruck S
FAU - Schwegmann-Wessels, Christel
AU  - Schwegmann-Wessels C
FAU - Kohl, Wiebke
AU  - Kohl W
FAU - Niedorf, Frank
AU  - Niedorf F
FAU - Stern, Michael
AU  - Stern M
FAU - Hewicker-Trautwein, Marion
AU  - Hewicker-Trautwein M
FAU - Bals, Robert
AU  - Bals R
FAU - Taylor, Geraldine
AU  - Taylor G
FAU - Braun, Armin
AU  - Braun A
FAU - Bicker, Gerd
AU  - Bicker G
FAU - Kietzmann, Manfred
AU  - Kietzmann M
FAU - Herrler, Georg
AU  - Herrler G
LA  - eng
GR  - BBS/E/I/00001052/BB_/Biotechnology and Biological Sciences Research 
      Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081203
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cells, Cultured
MH  - Epithelial Cells/*virology
MH  - Parainfluenza Virus 3, Bovine/*growth & development
MH  - Respiratory Mucosa/*virology
MH  - Respiratory Syncytial Virus, Bovine/*growth & development
PMC - PMC2643795
EDAT- 2008/12/05 09:00
MHDA- 2009/02/14 09:00
PMCR- 2009/08/01
CRDT- 2008/12/05 09:00
PHST- 2008/12/05 09:00 [pubmed]
PHST- 2009/02/14 09:00 [medline]
PHST- 2008/12/05 09:00 [entrez]
PHST- 2009/08/01 00:00 [pmc-release]
AID - 1271-08 [pii]
AID - 10.1128/JVI.01271-08 [doi]
PST - ppublish
SO  - J Virol. 2009 Feb;83(4):1962-8. doi: 10.1128/JVI.01271-08. Epub 2008 Dec 3.