PMID- 19053158
OWN - NLM
STAT- MEDLINE
DCOM- 20090728
LR  - 20151119
IS  - 1096-9888 (Electronic)
IS  - 1076-5174 (Linking)
VI  - 44
IP  - 5
DP  - 2009 May
TI  - Separation and determination of seleno amino acids using gas chromatography 
      hyphenated with inductively coupled plasma mass spectrometry after hollow fiber 
      liquid phase microextraction.
PG  - 605-12
LID - 10.1002/jms.1533 [doi]
AB  - A new derivatization-extraction method for preconcentration of seleno amino acids 
      using hollow fiber liquid phase microextraction (HF-LPME) was developed for the 
      separation and determination of seleno amino acids in biological samples by gas 
      chromatography-inductively coupled plasma mass spectrometry (GC-ICP-MS). 
      Derivatization was performed with ethyl chloroformate (ECF) to improve the 
      volatility of seleno amino acids. Parameters influencing microextraction, 
      including extraction solvent, pH of sample solution, extraction time, stirring 
      speed, and inorganic salt concentration have been investigated. Under the optimal 
      conditions, the limits of detection (LODs) obtained for Se-methyl-selenocysteine 
      (SeMeCys), selenomethionine (SeMet), and selenoethionine (SeEth) were 23, 15, and 
      11 ng Se l(-1), respectively. The relative standard deviations (RSDs) were 14.6%, 
      16.4%, and 19.4% for SeMeCys, SeMet, and SeEth (c = 1.0 ng ml(-1), n = 7), 
      respectively, and the RSDs for SeMeCys, SeMet could be improved obviously if 
      SeEth was utilized as the internal standard. The proposed method was applied for 
      the determination of seleno amino acids in extracts of garlic, cabbage, and 
      mushroom samples, and the recoveries for the spiked samples were in the range of 
      96.8-108% and 93.4-115% with and without the use of SeEth as internal standard. 
      The developed method was also applied to the analysis of SeMet in a certified 
      reference material of SELM-1 yeast and the determined value is in good agreement 
      with the certified value.
CI  - 2008 John Wiley & Sons, Ltd.
FAU - Duan, Jiankun
AU  - Duan J
AD  - Department of Chemistry, Wuhan University, Wuhan 430072, China.
FAU - Hu, Bin
AU  - Hu B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Mass Spectrom
JT  - Journal of mass spectrometry : JMS
JID - 9504818
RN  - 0 (Organoselenium Compounds)
RN  - 0CH9049VIS (Selenocysteine)
RN  - 3FPU23BG52 (Toluene)
RN  - 451W47IQ8X (Sodium Chloride)
RN  - 7V31YC746X (Chloroform)
RN  - 964MRK2PEL (Selenomethionine)
RN  - K848JZ4886 (Cysteine)
RN  - TWK220499Z (selenomethylselenocysteine)
RN  - WX1BN24WZT (Ethionine)
SB  - IM
MH  - Agaricales/chemistry
MH  - Brassica/chemistry
MH  - Chemical Fractionation/*methods
MH  - Chloroform/chemistry
MH  - Cysteine/analogs & derivatives/analysis
MH  - Ethionine/analogs & derivatives/analysis
MH  - Garlic/chemistry
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Organoselenium Compounds/*analysis
MH  - Selenocysteine/analogs & derivatives
MH  - Selenomethionine/analysis
MH  - Sensitivity and Specificity
MH  - Sodium Chloride/chemistry
MH  - Toluene/chemistry
EDAT- 2008/12/05 09:00
MHDA- 2009/07/29 09:00
CRDT- 2008/12/05 09:00
PHST- 2008/12/05 09:00 [pubmed]
PHST- 2009/07/29 09:00 [medline]
PHST- 2008/12/05 09:00 [entrez]
AID - 10.1002/jms.1533 [doi]
PST - ppublish
SO  - J Mass Spectrom. 2009 May;44(5):605-12. doi: 10.1002/jms.1533.