PMID- 19053858
OWN - NLM
STAT- MEDLINE
DCOM- 20090205
LR  - 20131121
IS  - 1557-7600 (Electronic)
IS  - 1096-620X (Linking)
VI  - 11
IP  - 4
DP  - 2008 Dec
TI  - Methionine supplementation accelerates oxidative stress and nuclear factor kappaB 
      activation in livers of C57BL/6 mice.
PG  - 667-74
LID - 10.1089/jmf.2007.0146 [doi]
AB  - The present study was designed to investigate whether hyperhomocysteinemia (HHcy) 
      induced by methionine supplementation promotes oxidative stress and nuclear 
      factor kappaB (NFkappaB) activation in livers of C57BL/6 mice when fed a 2% 
      methionine and low folate (1 mg/kg) diet for 12 weeks. Plasma homocysteine 
      concentrations of mice fed methionine were found to be 49 micromol/L by 12 weeks 
      of feeding, which was five times higher than that of controls. HHcy induced by 
      methionine feeding significantly increased oxidative stress, as measured by 
      thiobarbituric acid-reactive substances (P < .05) in livers. This was further 
      confirmed by lower levels of hepatic glutathione (P < .05) and elevated mRNA 
      expressions of hepatic antioxidative enzymes, such as Cu,Zn-superoxide dismutase, 
      catalase, glutathione reductase, and L-gulonolactone oxidase in methionine-fed 
      animals (P < .05). Hepatic function of mice fed methionine seems to be normal, 
      while hepatic triglyceride concentration was lowered by methionine feeding. 
      NFkappaB nuclear binding activities of livers were higher in the methionine group 
      than in the control group. The above results suggest that HHcy induced by 
      methionine may promote disturbances in lipid peroxidation and antioxidant 
      processes and be a pro-inflammatory mediator in livers of C57BL/6 mice.
FAU - Park, Chung-Mu
AU  - Park CM
AD  - Center of Smart Food & Drug, Biohealth Product Research Center and Food Science 
      Institute, Inje University, Gimhae, Republic of Korea.
FAU - Cho, Chung-Won
AU  - Cho CW
FAU - Rosenfeld, Michael E
AU  - Rosenfeld ME
FAU - Song, Young-Sun
AU  - Song YS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Med Food
JT  - Journal of medicinal food
JID - 9812512
RN  - 0 (Antioxidants)
RN  - 0 (Enzymes)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - 0 (Triglycerides)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - AE28F7PNPL (Methionine)
RN  - EC 1.- (Oxidoreductases)
SB  - IM
MH  - Animals
MH  - Antioxidants/metabolism
MH  - Cholesterol/blood
MH  - Dietary Supplements
MH  - Enzymes/genetics/metabolism
MH  - Homocysteine/blood
MH  - Hyperhomocysteinemia/chemically induced/*metabolism
MH  - Liver/enzymology/metabolism
MH  - Male
MH  - Methionine
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - NF-kappa B/*metabolism
MH  - *Oxidative Stress/drug effects
MH  - Oxidoreductases/metabolism
MH  - RNA, Messenger/metabolism
MH  - Thiobarbituric Acid Reactive Substances/metabolism
MH  - Triglycerides/blood
EDAT- 2008/12/05 09:00
MHDA- 2009/02/06 09:00
CRDT- 2008/12/05 09:00
PHST- 2008/12/05 09:00 [pubmed]
PHST- 2009/02/06 09:00 [medline]
PHST- 2008/12/05 09:00 [entrez]
AID - 10.1089/jmf.2007.0146 [doi]
PST - ppublish
SO  - J Med Food. 2008 Dec;11(4):667-74. doi: 10.1089/jmf.2007.0146.