PMID- 19054059
OWN - NLM
STAT- MEDLINE
DCOM- 20090709
LR  - 20090401
IS  - 1600-0625 (Electronic)
IS  - 0906-6705 (Linking)
VI  - 18
IP  - 4
DP  - 2009 Apr
TI  - The human skin/chick chorioallantoic membrane model accurately predicts the 
      potency of cosmetic allergens.
PG  - 409-13
LID - 10.1111/j.1600-0625.2008.00803.x [doi]
AB  - The current standard method for predicting contact allergenicity is the murine 
      local lymph node assay (LLNA). Public objection to the use of animals in testing 
      of cosmetics makes the development of a system that does not use sentient animals 
      highly desirable. The chorioallantoic membrane (CAM) of the chick egg has been 
      extensively used for the growth of normal and transformed mammalian tissues. The 
      CAM is not innervated, and embryos are sacrificed before the development of pain 
      perception. The aim of this study was to determine whether the sensitization 
      phase of contact dermatitis to known cosmetic allergens can be quantified using 
      CAM-engrafted human skin and how these results compare with published EC3 data 
      obtained with the LLNA. We studied six common molecules used in allergen testing 
      and quantified migration of epidermal Langerhans cells (LC) as a measure of their 
      allergic potency. All agents with known allergic potential induced statistically 
      significant migration of LC. The data obtained correlated well with published 
      data for these allergens generated using the LLNA test. The human-skin CAM model 
      therefore has great potential as an inexpensive, non-radioactive, in vivo 
      alternative to the LLNA, which does not require the use of sentient animals. In 
      addition, this system has the advantage of testing the allergic response of 
      human, rather than animal skin.
FAU - Slodownik, Dan
AU  - Slodownik D
AD  - Department of Dermatology, Hadassah-Hebrew University Medical Center, Jerusalem, 
      Israel. gbds@netvision.net.il
FAU - Grinberg, Igor
AU  - Grinberg I
FAU - Spira, Ram M
AU  - Spira RM
FAU - Skornik, Yehuda
AU  - Skornik Y
FAU - Goldstein, Ronald S
AU  - Goldstein RS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091023
PL  - Denmark
TA  - Exp Dermatol
JT  - Experimental dermatology
JID - 9301549
RN  - 0 (Allergens)
RN  - 0 (Cosmetics)
SB  - IM
MH  - Allergens/*immunology
MH  - Animals
MH  - Cell Movement/drug effects
MH  - Chick Embryo
MH  - Chorioallantoic Membrane/*immunology
MH  - Cosmetics/*adverse effects/pharmacology
MH  - Dermatitis, Allergic Contact/etiology/*immunology
MH  - Humans
MH  - Langerhans Cells/pathology
MH  - Models, Animal
MH  - *Models, Biological
MH  - Predictive Value of Tests
MH  - Skin/*immunology
MH  - Skin Tests
MH  - Transplants
EDAT- 2008/12/05 09:00
MHDA- 2009/07/10 09:00
CRDT- 2008/12/05 09:00
PHST- 2008/12/05 09:00 [pubmed]
PHST- 2009/07/10 09:00 [medline]
PHST- 2008/12/05 09:00 [entrez]
AID - EXD803 [pii]
AID - 10.1111/j.1600-0625.2008.00803.x [doi]
PST - ppublish
SO  - Exp Dermatol. 2009 Apr;18(4):409-13. doi: 10.1111/j.1600-0625.2008.00803.x. Epub 
      2009 Oct 23.