PMID- 19054610
OWN - NLM
STAT- MEDLINE
DCOM- 20090318
LR  - 20131121
IS  - 1872-7980 (Electronic)
IS  - 0304-3835 (Linking)
VI  - 276
IP  - 1
DP  - 2009 Apr 8
TI  - Okadaic acid promotes angiogenesis via activation of hypoxia-inducible factor-1.
PG  - 102-8
LID - 10.1016/j.canlet.2008.10.034 [doi]
AB  - Okadaic acid, a potent tumor promoter and an inhibitor of protein phosphatase 1 
      and 2A, has also been characterized as an angiogenic inducer in the 
      chorioallantoic membrane of the chick embryo. To elucidate the roles of okadaic 
      acid on angiogenic processes, we conducted in vitro angiogenesis assays. In this 
      study, we report that okadaic acid potently stimulated tube formation, migration, 
      and invasion of human umbilical vein endothelial cells. Moreover, okadaic acid 
      elevated the activities of hypoxia-inducible factor-1 (HIF-1), which is closely 
      related with the expression of vascular endothelial growth factor. Exposure to 
      okadaic acid markedly increased the HIF-1alpha protein level through 
      up-regulation of translation via activation of Akt and mTOR pathway. Taken 
      together, these results demonstrated that okadaic acid promotes angiogenesis 
      through stimulation of Akt mediated HIF-1alpha translation.
FAU - Kim, Young-Sun
AU  - Kim YS
AD  - Department of Anatomy and Neurobiology, Biomedical Science Institute, School of 
      Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea.
FAU - Ahn, Kook-Hee
AU  - Ahn KH
FAU - Kim, Seon-Ye
AU  - Kim SY
FAU - Jeong, Joo-Won
AU  - Jeong JW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081202
PL  - Ireland
TA  - Cancer Lett
JT  - Cancer letters
JID - 7600053
RN  - 0 (Angiogenesis Inducing Agents)
RN  - 0 (Carcinogens)
RN  - 0 (HIF1A protein, human)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 1W21G5Q4N2 (Okadaic Acid)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Angiogenesis Inducing Agents/*pharmacology
MH  - Blotting, Western
MH  - Carcinogens/pharmacology
MH  - Cell Movement/drug effects
MH  - Endothelial Cells/drug effects
MH  - Fluorescent Antibody Technique
MH  - Gene Expression Regulation/*drug effects
MH  - HeLa Cells
MH  - Humans
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/*drug effects/metabolism
MH  - Immunohistochemistry
MH  - Neovascularization, Physiologic/drug effects/*physiology
MH  - Okadaic Acid/*pharmacology
MH  - Protein Biosynthesis
MH  - Proto-Oncogene Proteins c-akt/biosynthesis/drug effects
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transfection
MH  - Umbilical Veins/drug effects
MH  - Vascular Endothelial Growth Factor A/biosynthesis/drug effects
EDAT- 2008/12/05 09:00
MHDA- 2009/03/19 09:00
CRDT- 2008/12/05 09:00
PHST- 2008/09/24 00:00 [received]
PHST- 2008/10/27 00:00 [revised]
PHST- 2008/10/27 00:00 [accepted]
PHST- 2008/12/05 09:00 [pubmed]
PHST- 2009/03/19 09:00 [medline]
PHST- 2008/12/05 09:00 [entrez]
AID - S0304-3835(08)00859-8 [pii]
AID - 10.1016/j.canlet.2008.10.034 [doi]
PST - ppublish
SO  - Cancer Lett. 2009 Apr 8;276(1):102-8. doi: 10.1016/j.canlet.2008.10.034. Epub 
      2008 Dec 2.