PMID- 1905567
OWN - NLM
STAT- MEDLINE
DCOM- 19910807
LR  - 20201209
IS  - 0863-4106 (Print)
IS  - 0863-4106 (Linking)
VI  - 137
IP  - 1
DP  - 1991
TI  - Electron microscopic localization of acridine orange binding to euchromatin in 
      human neuroblastoma cells.
PG  - 20-8
AB  - The purpose of the present study was to examine the distribution pattern of 
      acridine orange (AO) chromatin interaction products (AOCI) in human neuroblastoma 
      IMR-32 cells and to test whether AO labeling is correlated with BrdU 
      incorporation, and immunohistochemical localization of DNA polymerase alpha, and 
      human N-myc-gene product. Effects of aphidicolin, alpha-amanitin, and actinomycin 
      D on visualization of AO binding to euchromatin and on N-myc-gene expression were 
      also examined. About 25% of the cell nuclei in logarithmic growth phase were 
      immunohistochemically demonstrated to be labeled with BrdU after incubation at 37 
      degrees for 30 min, indicating cells in DNA synthesis. Most of the cell nuclei 
      showed positive immunoreactivity to DNA polymerase alpha, while human N-myc gene 
      product was found in about 60-80% of the cell nuclei. Electron microscopic 
      studies revealed that about 25% of neuroblastoma cells showed characteristic AOCI 
      within cell nuclei. In the presence of aphidicolin, alpha-amanitin, and 
      actinomycin D, positive cells for N-myc gene product decreased markedly. 
      Percentages of AO positive cells and numbers of AOCI per cell nucleus also showed 
      a marked decrease. But northern blot analysis demonstrated that the expression 
      level of N-myc gene was only repressed by the transcriptional inhibitors 
      alpha-amanitin and actinomycin D. However, no repression was caused by 
      aphidicolin. The present and previous studies of the authors suggest that the 
      ultracytochemical AO method may be indicative for conformational changes of 
      chromatin of cells confined to the cell cycle. Inhibitors of RNA and DNA 
      synthesis then may change the conformational state of chromatin.(ABSTRACT 
      TRUNCATED AT 250 WORDS)
FAU - Hiroi, M
AU  - Hiroi M
AD  - Department of Pathology, Kochi Medical School, Nankoku, Japan.
FAU - Moriki, T
AU  - Moriki T
FAU - Taniguchi, T
AU  - Taniguchi T
FAU - Yamane, T
AU  - Yamane T
FAU - Lehmann, R
AU  - Lehmann R
FAU - Hara, H
AU  - Hara H
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Zentralbl Pathol
JT  - Zentralblatt fur Pathologie
JID - 9105594
RN  - 0 (Amanitins)
RN  - 0 (Chromatin)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Diterpenes)
RN  - 0 (Euchromatin)
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 1CC1JFE158 (Dactinomycin)
RN  - 38966-21-1 (Aphidicolin)
RN  - EC 2.7.7.7 (DNA Polymerase II)
RN  - F30N4O6XVV (Acridine Orange)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Acridine Orange/analysis/*metabolism
MH  - Amanitins/pharmacology
MH  - Aphidicolin
MH  - Blotting, Northern
MH  - Bromodeoxyuridine/metabolism
MH  - Cell Nucleus/drug effects/metabolism/ultrastructure
MH  - Chromatin/*metabolism
MH  - DNA Polymerase II/analysis/antagonists & inhibitors
MH  - DNA, Neoplasm/analysis/biosynthesis
MH  - Dactinomycin/pharmacology
MH  - Diploidy
MH  - Diterpenes/pharmacology
MH  - Euchromatin
MH  - Flow Cytometry
MH  - Gene Expression Regulation, Neoplastic
MH  - Genes, myc
MH  - Humans
MH  - Immunohistochemistry
MH  - Microscopy, Electron
MH  - Neuroblastoma/genetics/*metabolism
MH  - Proto-Oncogene Proteins c-myc/biosynthesis
MH  - Tumor Cells, Cultured
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
PST - ppublish
SO  - Zentralbl Pathol. 1991;137(1):20-8.