PMID- 19056352
OWN - NLM
STAT- MEDLINE
DCOM- 20090227
LR  - 20211020
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 379
IP  - 3
DP  - 2009 Feb 13
TI  - 4-HNE inhibits tube formation and up-regulates chondromodulin-I in human 
      endothelial cells.
PG  - 654-8
LID - 10.1016/j.bbrc.2008.11.095 [doi]
AB  - The aim of the present study was to investigate the effects of 
      4-hydroxy-2-nonenal (4-HNE) on tube formation by human bone marrow endothelial 
      cells (HBMEC). We found that 4-HNE at physiologically achievable concentrations 
      (5 and 10 microM) inhibited the formation of tubes. Western blot analysis 
      revealed that inhibition of tube formation by 4-HNE was associated with increased 
      expression of chondromodulin-I (CHM-I), a protein with well-known anti-angiogenic 
      properties. Cell viability assays showed that 4-HNE at concentrations of 10 
      microM or less did not cause HBMEC cell death. Luciferase reporter assays did not 
      show any inducing effect of 4-HNE on the promoter activity of human CHM-I gene 
      indicating that post-transcriptional or post-translational modifications may 
      account for the up-regulation of CHM-I. Collectively, the results of the present 
      study show for the first time that 4-HNE inhibits tube formation by HBMECs 
      indicating a potential anti-angiogenic activity of 4-HNE. This inhibition occurs 
      at least in part via 4-HNE-induced CHM-I protein expression.
FAU - Stagos, Dimitrios
AU  - Stagos D
AD  - Molecular Toxicology & Environmental Health Sciences Program, Department of 
      Pharmaceutical Sciences, University of Colorado-Denver, 12700 E 19th Avenue, 
      Aurora, CO 80045, USA.
FAU - Zhou, Hongfei
AU  - Zhou H
FAU - Ross, David
AU  - Ross D
FAU - Vasiliou, Vasilis
AU  - Vasiliou V
LA  - eng
GR  - ES 09554/ES/NIEHS NIH HHS/United States
GR  - R29 EY011490/EY/NEI NIH HHS/United States
GR  - R01 EY011490/EY/NEI NIH HHS/United States
GR  - R01 EY011490-10/EY/NEI NIH HHS/United States
GR  - EY 11490/EY/NEI NIH HHS/United States
GR  - R01 ES009554-07/ES/NIEHS NIH HHS/United States
GR  - R01 ES009554/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20081203
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Aldehydes)
RN  - 0 (Cysteine Proteinase Inhibitors)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Proteins)
RN  - 136362-10-2 (CNMD protein, human)
RN  - K1CVM13F96 (4-hydroxy-2-nonenal)
SB  - IM
MH  - Aldehydes/*pharmacology
MH  - Bone Marrow Cells/*drug effects/metabolism
MH  - Cells, Cultured
MH  - Cysteine Proteinase Inhibitors/*pharmacology
MH  - Endothelial Cells/*drug effects/metabolism
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins/*biosynthesis/genetics
MH  - Membrane Proteins/*biosynthesis/genetics
MH  - Neovascularization, Physiologic/*drug effects
MH  - Promoter Regions, Genetic/drug effects
MH  - Up-Regulation
PMC - PMC2757412
MID - NIHMS95188
EDAT- 2008/12/06 09:00
MHDA- 2009/02/28 09:00
PMCR- 2010/02/13
CRDT- 2008/12/06 09:00
PHST- 2008/11/19 00:00 [received]
PHST- 2008/11/20 00:00 [accepted]
PHST- 2008/12/06 09:00 [pubmed]
PHST- 2009/02/28 09:00 [medline]
PHST- 2008/12/06 09:00 [entrez]
PHST- 2010/02/13 00:00 [pmc-release]
AID - S0006-291X(08)02306-1 [pii]
AID - 10.1016/j.bbrc.2008.11.095 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2009 Feb 13;379(3):654-8. doi: 
      10.1016/j.bbrc.2008.11.095. Epub 2008 Dec 3.