PMID- 19057860 OWN - NLM STAT- MEDLINE DCOM- 20090319 LR - 20211020 IS - 1064-3745 (Print) IS - 1064-3745 (Linking) VI - 454 DP - 2008 TI - The preparation of chicken tracheal organ cultures for virus isolation, propagation, and titration. PG - 103-7 LID - 10.1007/978-1-59745-181-9_9 [doi] AB - Chicken tracheal organ cultures (TOCs), comprising transverse sections of chick embryo trachea with beating cilia, have proved useful in the isolation of several respiratory viruses and as a viral assay system, using ciliostasis as the criterion for infection. A simple technique for the preparation of chicken tracheal organ cultures in glass test tubes, in which virus growth and ciliostasis can be readily observed, is described. FAU - Jones, Brenda V AU - Jones BV AD - Institute for Animal Health, Compton, Newbury, Berkshire, UK. FAU - Hennion, Ruth M AU - Hennion RM LA - eng PT - Journal Article PL - United States TA - Methods Mol Biol JT - Methods in molecular biology (Clifton, N.J.) JID - 9214969 SB - IM MH - Animals MH - Chickens/*virology MH - Coronavirus/growth & development/isolation & purification MH - Infectious bronchitis virus/growth & development/isolation & purification MH - Organ Culture Techniques/methods MH - Trachea/*virology PMC - PMC7121057 EDAT- 2008/12/06 09:00 MHDA- 2009/03/20 09:00 PMCR- 2020/04/03 CRDT- 2008/12/06 09:00 PHST- 2008/12/06 09:00 [pubmed] PHST- 2009/03/20 09:00 [medline] PHST- 2008/12/06 09:00 [entrez] PHST- 2020/04/03 00:00 [pmc-release] AID - 9 [pii] AID - 10.1007/978-1-59745-181-9_9 [doi] PST - ppublish SO - Methods Mol Biol. 2008;454:103-7. doi: 10.1007/978-1-59745-181-9_9.