PMID- 19057971
OWN - NLM
STAT- MEDLINE
DCOM- 20091007
LR  - 20211020
IS  - 1543-706X (Electronic)
IS  - 1071-2690 (Linking)
VI  - 45
IP  - 1-2
DP  - 2009 Jan-Feb
TI  - Simultaneous isolation of vascular endothelial cells and mesenchymal stem cells 
      from the human umbilical cord.
PG  - 23-7
LID - 10.1007/s11626-008-9155-4 [doi]
AB  - The umbilical cord represents the link between mother and fetus during pregnancy. 
      This cord is usually discarded as a biological waste after the child's birth; 
      however, its importance as a "store house" of stem cells has been explored 
      recently. We developed a method of simultaneous isolation of endothelial cells 
      (ECs) from the vein and mesenchymal stem cells from umbilical cord Wharton's 
      jelly of the same cord. The isolation protocol has been simplified, modified, and 
      improvised with respect to choice of enzyme and enzyme mixture, digestion time, 
      cell yield, cell growth, and culture medium. Isolated human umbilical vascular 
      ECs (hUVECs) were positive for von-Willibrand factor, a classical endothelial 
      marker, and could form capillary-like structures when seeded on Matrigel, thus 
      proving their functionality. The isolated human umbilical cord mesenchymal stem 
      cells (hUCMSCs) were found positive for CD44, CD90, CD 73, and CD117 and were 
      found negative for CD33, CD34, CD45, and CD105 surface markers; they were also 
      positive for cytoskeleton markers of smooth muscle actin and vimentin. The 
      hUCMSCs showed multilineage differentiation potential and differentiated into 
      adipogenic, chondrogenic, osteogenic, and neuronal lineages under influence of 
      lineage specific differentiation medium. Thus, isolating endothelial cells as 
      well as mesenchymal cells from the same umbilical cord could lead to complete 
      utilization of the available tissue for the tissue engineering and cell therapy.
FAU - Kadam, Sachin S
AU  - Kadam SS
AD  - National Center for Cell Science, Ganeshkhind, Pune 411007, India.
FAU - Tiwari, Shubha
AU  - Tiwari S
FAU - Bhonde, Ramesh R
AU  - Bhonde RR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081205
PL  - Germany
TA  - In Vitro Cell Dev Biol Anim
JT  - In vitro cellular & developmental biology. Animal
JID - 9418515
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation)
SB  - IM
MH  - Antigens, CD/analysis
MH  - Antigens, Differentiation/analysis
MH  - Cell Culture Techniques/*methods
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Endothelial Cells/*cytology
MH  - Female
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology/physiology
MH  - Microscopy, Phase-Contrast
MH  - Pregnancy
MH  - Umbilical Cord/*cytology
EDAT- 2008/12/06 09:00
MHDA- 2009/10/08 06:00
CRDT- 2008/12/06 09:00
PHST- 2008/07/09 00:00 [received]
PHST- 2008/10/13 00:00 [accepted]
PHST- 2008/12/06 09:00 [pubmed]
PHST- 2009/10/08 06:00 [medline]
PHST- 2008/12/06 09:00 [entrez]
AID - 10.1007/s11626-008-9155-4 [doi]
PST - ppublish
SO  - In Vitro Cell Dev Biol Anim. 2009 Jan-Feb;45(1-2):23-7. doi: 
      10.1007/s11626-008-9155-4. Epub 2008 Dec 5.