PMID- 19058870
OWN - NLM
STAT- MEDLINE
DCOM- 20090709
LR  - 20181201
IS  - 1872-8332 (Electronic)
IS  - 0169-5002 (Linking)
VI  - 64
IP  - 3
DP  - 2009 Jun
TI  - Epidermal growth factor receptor gene amplification in surgical resected Japanese 
      lung cancer.
PG  - 295-300
LID - 10.1016/j.lungcan.2008.10.005 [doi]
AB  - To evaluate the epidermal growth factor receptor (EGFR) protein expression and 
      increased copy number as predictors of clinical outcome in patients with 
      non-small-cell lung cancer (NSCLC), we have performed fluorescence in situ 
      hybridization (FISH) and immunohistochemistry (IHC). We investigated the EGFR 
      increased copy number and EGFR protein expression statuses in 109 surgically 
      treated NSCLC cases. The presence or absence of EGFR mutations of kinase domains 
      was analyzed by genotyping analysis and sequences, and already reported. EGFR 
      increased copy number was defined as Cappuzzo et al. criteria. FISH positive was 
      found from 36/109 (33.0%) lung cancer patients, including 30 high polysomy cases 
      and 6 gene amplification cases. FISH-positive cases were significantly correlated 
      with worse prognosis (log-rank test p=0.0097). Within EGFR-mutant patients 
      (n=55), FISH-positive cases were also correlated with poor prognosis (p=0.0255). 
      FISH-negative tumors were found to be more frequently well-differentiated 
      histology. Smoking status (never smoker vs. smoker, p=0.1510), and gender 
      (p=0.5248) did not correlated with FISH positive. EGFR IHC results were 
      correlated with FISH results (p=0.004), but not correlated with prognosis 
      (p=0.2815). Although EGFR FISH-positive rate did not correlated with EGFR 
      mutation (p=0.1973), EGFR polysomy or amplification cases were correlated with 
      EGFR mutations (p=0.0023). In conclusion, the EGFR FISH-positive rate in Japanese 
      patients with NSCLC was similar to rates in Western populations, unlike the 
      higher frequencies of EGFR mutation in East Asians. A high EGFR gene copy number 
      might have shorter survival in NSCLC.
FAU - Sasaki, Hidefumi
AU  - Sasaki H
AD  - Department of Surgery II, Nagoya City University Medical School, Nagoya 467-8601, 
      Japan. hisasaki@med.nagoya-cu.ac.jp
FAU - Shimizu, Shigeki
AU  - Shimizu S
FAU - Okuda, Katsuhiro
AU  - Okuda K
FAU - Kawano, Osamu
AU  - Kawano O
FAU - Yukiue, Haruhiro
AU  - Yukiue H
FAU - Yano, Motoki
AU  - Yano M
FAU - Fujii, Yoshitaka
AU  - Fujii Y
LA  - eng
PT  - Journal Article
DEP - 20081205
PL  - Ireland
TA  - Lung Cancer
JT  - Lung cancer (Amsterdam, Netherlands)
JID - 8800805
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Aged
MH  - Carcinoma, Non-Small-Cell Lung/*diagnosis/*genetics/surgery
MH  - Cell Differentiation
MH  - ErbB Receptors/*genetics/metabolism
MH  - Female
MH  - *Gene Amplification
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Japan
MH  - Lung Neoplasms/*diagnosis/*genetics/surgery
MH  - Male
MH  - Middle Aged
MH  - Mutation
MH  - Prognosis
MH  - Risk Factors
MH  - Sex Factors
MH  - Smoking
EDAT- 2008/12/09 09:00
MHDA- 2009/07/10 09:00
CRDT- 2008/12/09 09:00
PHST- 2008/06/26 00:00 [received]
PHST- 2008/08/04 00:00 [revised]
PHST- 2008/10/06 00:00 [accepted]
PHST- 2008/12/09 09:00 [pubmed]
PHST- 2009/07/10 09:00 [medline]
PHST- 2008/12/09 09:00 [entrez]
AID - S0169-5002(08)00516-3 [pii]
AID - 10.1016/j.lungcan.2008.10.005 [doi]
PST - ppublish
SO  - Lung Cancer. 2009 Jun;64(3):295-300. doi: 10.1016/j.lungcan.2008.10.005. Epub 
      2008 Dec 5.