PMID- 19059495
OWN - NLM
STAT- MEDLINE
DCOM- 20090605
LR  - 20161020
IS  - 1769-714X (Electronic)
IS  - 1286-4579 (Linking)
VI  - 11
IP  - 2
DP  - 2009 Feb
TI  - Activation of HIV-1 Gag-specific CD8+ T cells by yeast-derived VLP-pulsed 
      dendritic cells is influenced by the level of mannose on the VLP antigen.
PG  - 191-7
LID - 10.1016/j.micinf.2008.11.004 [doi]
AB  - Dendritic cells (DCs) are professional antigen-presenting cells that possess a 
      unique capacity to cross-present exogenous antigens efficiently to CD8(+) T 
      cells. We previously demonstrated that monocyte-derived DCs (MDDCs) pulsed with 
      yeast-derived HIV-1 Gag virus-like particles (VLPs) were able to activate 
      Gag-specific CD8(+) T cells from HIV-1-infected individuals. Yeast VLPs are 
      abundantly mannosylated (high-mannose type: HmVLPs) and are highly immunogenic. 
      Because lectin receptors are shown to negatively regulate Th1 responses, we 
      investigated the relationship between VLP mannosylation level and MDDC 
      cross-presentation activity. Poorly mannosylated VLPs (low-mannose type: LmVLPs) 
      were prepared using a yeast mnn9 mutant strain that lacks a core mannosylation 
      enzyme. We found that MDDCs pulsed with LmVLPs activated Gag-specific T cells 
      more strongly than those pulsed with HmVLPs. However, MDDCs showed similar 
      antigen uptake and intracellular transport of both types of VLPs. Interestingly, 
      LmVLPs induced IL-12 production slightly more than HmVLPs (yet statistically 
      significant). Furthermore, the level of LPS-induced IL-10 production was enhanced 
      by pulsing with HmVLPs, but not with LmVLPs. These results indicate that lectin 
      receptors recognizing mannose may influence the Th1/Th2 balance of the immune 
      response, resulting in reduced efficiency of CD8(+) T cell activation by a 
      heavily mannosylated antigen presented by DCs.
FAU - Mizukoshi, Fuminori
AU  - Mizukoshi F
AD  - Department of Immunology, National Institute of Infectious Diseases, Toyama 
      1-23-1 Shinjuku-ku, Tokyo, Japan.
FAU - Yamamoto, Takuya
AU  - Yamamoto T
FAU - Mitsuki, Yu-Ya
AU  - Mitsuki YY
FAU - Terahara, Kazutaka
AU  - Terahara K
FAU - Kawana-Tachikawa, Ai
AU  - Kawana-Tachikawa A
FAU - Kobayashi, Kazuo
AU  - Kobayashi K
FAU - Iwamoto, Aikichi
AU  - Iwamoto A
FAU - Morikawa, Yuko
AU  - Morikawa Y
FAU - Tsunetsugu-Yokota, Yasuko
AU  - Tsunetsugu-Yokota Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081124
PL  - France
TA  - Microbes Infect
JT  - Microbes and infection
JID - 100883508
RN  - 0 (Virosomes)
RN  - 0 (gag Gene Products, Human Immunodeficiency Virus)
RN  - PHA4727WTP (Mannose)
SB  - IM
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology
MH  - HIV-1/*immunology
MH  - Humans
MH  - Mannose/*analysis
MH  - Saccharomyces cerevisiae/genetics/metabolism
MH  - Virosomes/*chemistry/genetics/*immunology
MH  - gag Gene Products, Human Immunodeficiency Virus/genetics/*immunology
EDAT- 2008/12/09 09:00
MHDA- 2009/06/06 09:00
CRDT- 2008/12/09 09:00
PHST- 2008/10/03 00:00 [received]
PHST- 2008/11/10 00:00 [revised]
PHST- 2008/11/12 00:00 [accepted]
PHST- 2008/12/09 09:00 [pubmed]
PHST- 2009/06/06 09:00 [medline]
PHST- 2008/12/09 09:00 [entrez]
AID - S1286-4579(08)00310-9 [pii]
AID - 10.1016/j.micinf.2008.11.004 [doi]
PST - ppublish
SO  - Microbes Infect. 2009 Feb;11(2):191-7. doi: 10.1016/j.micinf.2008.11.004. Epub 
      2008 Nov 24.