PMID- 19059946
OWN - NLM
STAT- MEDLINE
DCOM- 20090825
LR  - 20090224
IS  - 1460-2350 (Electronic)
IS  - 0268-1161 (Linking)
VI  - 24
IP  - 3
DP  - 2009 Mar
TI  - The experience of two European preimplantation genetic diagnosis centres on human 
      leukocyte antigen typing.
PG  - 732-40
LID - 10.1093/humrep/den423 [doi]
AB  - BACKGROUND: Two European centres report on human leukocyte antigen (HLA) typing 
      of preimplantation embryos for haematopoietic stem cell (HSC) transplantation: 
      'UZ Brussel' in Brussels and 'Genoma' in Rome. Both centres have 6 years' 
      experience with technical and clinical aspects of this type of genetic analysis 
      on single blastomeres. METHODS: Both centres apply a similar technique for 
      preimplantation HLA typing using short tandem repeats linked to the HLA locus in 
      multiplex PCR for haplotyping. RESULTS: At present, a conclusive HLA diagnosis 
      could be assured in 92.8% and 90.3% of the embryos at UZ Brussel and at Genoma, 
      respectively. The implantation rates were 32.4% and 28.2%, respectively, and the 
      birth rates per cycle were 9.4% and 18.6%, respectively. The HLA programme at UZ 
      Brussel and at Genoma resulted in the birth of 9 babies and 3 successful HSC 
      transplantations, and 42 babies and 7 successful HSC transplantations, 
      respectively, so far. CONCLUSIONS: Drastic embryo selection for preimplantation 
      HLA typing (in theory 1/4 for HLA, 1/8 for HLA in combination with sexing for 
      X-linked recessive diseases, 3/16 for HLA in combination with autosomal recessive 
      disorders) resulted overall in the birth of 51 babies (15.9% live birth rate per 
      started cycle) in two European centres.
FAU - Van de Velde, Hilde
AU  - Van de Velde H
AD  - Centre for Reproductive Medicine, UZ Brussel, Laarbeeklaan 101, 1090 Brussels, 
      Belgium. hilde.vandevelde@uzbrussel.be
FAU - De Rycke, Martine
AU  - De Rycke M
FAU - De Man, Caroline
AU  - De Man C
FAU - De Hauwere, Kim
AU  - De Hauwere K
FAU - Fiorentino, Francesco
AU  - Fiorentino F
FAU - Kahraman, Semra
AU  - Kahraman S
FAU - Pennings, Guido
AU  - Pennings G
FAU - Verpoest, Willem
AU  - Verpoest W
FAU - Devroey, Paul
AU  - Devroey P
FAU - Liebaers, Inge
AU  - Liebaers I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081205
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Adult
MH  - Alleles
MH  - Belgium
MH  - Female
MH  - Genetic Markers
MH  - Haplotypes
MH  - Hematopoietic Stem Cell Transplantation/*methods
MH  - Histocompatibility Testing/*methods
MH  - Humans
MH  - Italy
MH  - Male
MH  - Maternal Age
MH  - Pregnancy
MH  - Pregnancy Outcome
MH  - Preimplantation Diagnosis/*methods
EDAT- 2008/12/09 09:00
MHDA- 2009/08/26 09:00
CRDT- 2008/12/09 09:00
PHST- 2008/12/09 09:00 [pubmed]
PHST- 2009/08/26 09:00 [medline]
PHST- 2008/12/09 09:00 [entrez]
AID - den423 [pii]
AID - 10.1093/humrep/den423 [doi]
PST - ppublish
SO  - Hum Reprod. 2009 Mar;24(3):732-40. doi: 10.1093/humrep/den423. Epub 2008 Dec 5.