PMID- 19062520
OWN - NLM
STAT- MEDLINE
DCOM- 20090115
LR  - 20131121
IS  - 0041-3771 (Print)
IS  - 0041-3771 (Linking)
VI  - 50
IP  - 10
DP  - 2008
TI  - [Changes in matrix metalloproteinases activities in normal and transformed mouse 
      fibroblasts under effect of antioxidants].
PG  - 877-81
AB  - The effect of two antioxidants on the activities of matrix metalloproteinases 
      (MMP) secreted by normal (3T3) and transformed (3T3-SV40) mouse fibroblasts was 
      examined. We compared the effect of N-acetylcystein (NAC) and alpha-lipoic acid 
      (ALA) on two gelatinases, MMP-2 and MMP-9. Gel zymography demonstrated that 
      activity of MMP-2 was higher in normal 3T3 cells, and MMP-9 activity was higher 
      in transformed 3T3-SV40 cells. NAC action for 2-6 hours completely inhibited 
      MMP-2 and MMP-9 activity in both cell lines. The inhibitory effect almost did not 
      depend on NAC concentration at the range of 1-10 mM. ALA (1.2 mM) affected the 
      cells not so dramatically. ALA decreased the MMP-2 activity in both cellular 
      types. As to MMP-9 activity, it decreased in 3T3 cells and slightly increased in 
      3T3-SV40 cells in the presence of ALA. The activity of membrane bound and 
      intracellular MMP was not changed under the same conditions. In conclusion, an 
      altered activity of MMP in the presence of an antioxidant may influence the 
      intracellular signalling and cell functions.
FAU - Voronkina, I V
AU  - Voronkina IV
FAU - Kirpichnikova, K M
AU  - Kirpichnikova KM
FAU - Smagina, L V
AU  - Smagina LV
FAU - Gamalii, I A
AU  - Gamalii IA
LA  - rus
PT  - Comparative Study
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Russia (Federation)
TA  - Tsitologiia
JT  - Tsitologiia
JID - 0417363
RN  - 0 (Antioxidants)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - 73Y7P0K73Y (Thioctic Acid)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - 3T3 Cells
MH  - Acetylcysteine/*pharmacology
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Cell Line, Transformed
MH  - Cell Transformation, Viral/drug effects
MH  - Enzyme Inhibitors/*pharmacology
MH  - Fibroblasts/drug effects/enzymology
MH  - Matrix Metalloproteinase 2/metabolism
MH  - Matrix Metalloproteinase 9/metabolism
MH  - *Matrix Metalloproteinase Inhibitors
MH  - Mice
MH  - Thioctic Acid/*pharmacology
EDAT- 2008/12/10 09:00
MHDA- 2009/01/16 09:00
CRDT- 2008/12/10 09:00
PHST- 2008/12/10 09:00 [pubmed]
PHST- 2009/01/16 09:00 [medline]
PHST- 2008/12/10 09:00 [entrez]
PST - ppublish
SO  - Tsitologiia. 2008;50(10):877-81.