PMID- 19063994
OWN - NLM
STAT- MEDLINE
DCOM- 20090428
LR  - 20141120
IS  - 1537-1891 (Print)
IS  - 1537-1891 (Linking)
VI  - 50
IP  - 3-4
DP  - 2009 Mar-Apr
TI  - Effect of Benincasa hispida Cogniaux on high glucose-induced vascular 
      inflammation of human umbilical vein endothelial cells.
PG  - 116-22
LID - 10.1016/j.vph.2008.11.007 [doi]
AB  - Vascular inflammation is an important factor which can promote diabetic 
      complications. Preliminary investigations of several crude plant extracts 
      including aqueous extract of Benincasa hispida Cogniaux exhibit anti-inflammatory 
      properties. This study investigates the mechanism of anti-vascular inflammatory 
      activity of an aqueous extract of B. hispida Cogniaux (ABH) in human umbilical 
      vein endothelial cells (HUVECs). The study was performed on HUVECs that were 
      pretreated with various concentrations (1-20 microg/ml) of ABH before exposure 
      with high glucose (25 mM) for 48 h. Cell ELISA and Western blot analysis showed 
      that ABH inhibited high glucose-induced cell adhesion molecules (CAMs) surface 
      and protein expression, resulting in reduced adhesion of U937 monocytes. ABH also 
      inhibited the mRNA expression level of monocyte chemoattractant protein-1 (MCP-1) 
      and interleukin-8 (IL-8). High glucose-induced ROS production was inhibited by 
      treatment of ABH. We observed that pretreatment with HUVECs with ABH blocks 
      NF-kappaB activation via blocking phosphorylation and degradation of its 
      inhibitory protein, IkappaB-alpha. ABH also reduced NF-kB promoter activity. 
      These results suggest that ABH reduces high glucose-induced CAMs activation by 
      inhibiting monocyte adhesion, ROS, and NF-kappaB in HUVECs.
FAU - Moon, Mi Kyoung
AU  - Moon MK
AD  - Professional Graduate School of Oriental Medicine Wonkwang University, Iksan, 
      Jeonbuk, 570-749, Republic of Korea.
FAU - Kang, Dae Gill
AU  - Kang DG
FAU - Lee, Yun Jung
AU  - Lee YJ
FAU - Kim, Jin Sook
AU  - Kim JS
FAU - Lee, Ho Sub
AU  - Lee HS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081127
PL  - United States
TA  - Vascul Pharmacol
JT  - Vascular pharmacology
JID - 101130615
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Interleukin-8)
RN  - 0 (NF-kappa B)
RN  - 0 (Plant Extracts)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reactive Oxygen Species)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Cell Adhesion/drug effects
MH  - Cell Adhesion Molecules/metabolism
MH  - Cells, Cultured
MH  - *Cucurbitaceae
MH  - Endothelium, Vascular/drug effects/*immunology
MH  - Female
MH  - Glucose/administration & dosage/*pharmacology
MH  - Humans
MH  - In Vitro Techniques
MH  - Interleukin-8/metabolism
MH  - Monocytes/drug effects
MH  - NF-kappa B/biosynthesis
MH  - Plant Extracts/*pharmacology
MH  - Pregnancy
MH  - RNA, Messenger/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Umbilical Veins/*drug effects/*immunology/metabolism
MH  - Vasculitis/chemically induced/*prevention & control
EDAT- 2008/12/10 09:00
MHDA- 2009/04/29 09:00
CRDT- 2008/12/10 09:00
PHST- 2008/06/16 00:00 [received]
PHST- 2008/11/04 00:00 [revised]
PHST- 2008/11/14 00:00 [accepted]
PHST- 2008/12/10 09:00 [pubmed]
PHST- 2009/04/29 09:00 [medline]
PHST- 2008/12/10 09:00 [entrez]
AID - S1537-1891(08)00144-4 [pii]
AID - 10.1016/j.vph.2008.11.007 [doi]
PST - ppublish
SO  - Vascul Pharmacol. 2009 Mar-Apr;50(3-4):116-22. doi: 10.1016/j.vph.2008.11.007. 
      Epub 2008 Nov 27.