PMID- 19064723
OWN - NLM
STAT- MEDLINE
DCOM- 20090410
LR  - 20220225
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Linking)
VI  - 113
IP  - 9
DP  - 2009 Feb 26
TI  - Activated T cells recruit exosomes secreted by dendritic cells via LFA-1.
PG  - 1977-81
LID - 10.1182/blood-2008-08-174094 [doi]
AB  - Dendritic cells (DCs) are known to secrete exosomes that transfer membrane 
      proteins, like major histocompatibility complex class II, to other DCs. 
      Intercellular transfer of membrane proteins is also observed during cognate 
      interactions between DCs and CD4(+) T cells. The acquired proteins are functional 
      and play a role in regulation of immune responses. How membrane protein transfer 
      is achieved and regulated is unclear. Here we show that T cells can recruit major 
      histocompatibility complex class II-containing DC exosomes secreted in the 
      extracellular milieu during cognate DC-T-cell interactions. Recruitment of these 
      exosomes required T-cell activation and was dependent on leukocyte 
      function-associated antigen-1 (LFA-1) rather than on T-cell receptor specificity. 
      Indeed, inducing a high-affinity state of LFA-1 on resting T cells was sufficient 
      to provoke exosome binding. These results imply that DC exosomes secreted in the 
      extracellular milieu during cognate T-cell-DC interactions are targeted to T 
      cells activated in that microenvironment.
FAU - Nolte-'t Hoen, Esther N M
AU  - Nolte-'t Hoen EN
AD  - Faculty of Veterinary Medicine, Department of Biochemistry & Cell Biology, 
      Utrecht University, Utrecht, The Netherlands.
FAU - Buschow, Sonja I
AU  - Buschow SI
FAU - Anderton, Stephen M
AU  - Anderton SM
FAU - Stoorvogel, Willem
AU  - Stoorvogel W
FAU - Wauben, Marca H M
AU  - Wauben MH
LA  - eng
GR  - G0801924/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
DEP - 20081208
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Lymphocyte Function-Associated Antigen-1)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
EIN - Blood. 2009 Sep 24;114(13):2851
MH  - Animals
MH  - CD4-Positive T-Lymphocytes/immunology/*metabolism
MH  - Cattle
MH  - Cell Communication/immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/*metabolism
MH  - Exosomes/*metabolism
MH  - Genes, p53/physiology
MH  - Intercellular Adhesion Molecule-1/metabolism
MH  - Lymphocyte Activation/*physiology
MH  - Lymphocyte Function-Associated Antigen-1/metabolism/*physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Protein Binding
EDAT- 2008/12/10 09:00
MHDA- 2009/04/11 09:00
CRDT- 2008/12/10 09:00
PHST- 2008/12/10 09:00 [pubmed]
PHST- 2009/04/11 09:00 [medline]
PHST- 2008/12/10 09:00 [entrez]
AID - S0006-4971(20)37639-4 [pii]
AID - 10.1182/blood-2008-08-174094 [doi]
PST - ppublish
SO  - Blood. 2009 Feb 26;113(9):1977-81. doi: 10.1182/blood-2008-08-174094. Epub 2008 
      Dec 8.