PMID- 19065537
OWN - NLM
STAT- MEDLINE
DCOM- 20090325
LR  - 20081209
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 25
IP  - 6
DP  - 2008 Dec
TI  - [Detection of the SRY gene by primed in situ labeling].
PG  - 701-3
AB  - OBJECTIVE: To establish a primed in situ labeling (PRINS) technique which can be 
      more effective in detection of single copy gene. METHODS: On the basis of 
      traditional PRINS, new reagents and procedures, such as TaqStart antibody, four 
      primers of the sex determining region Y (SRY) gene and TSA(TM) Biotin System were 
      included in detection of the SRY gene. Meanwhile, fluorescence in situ 
      hybridization(FISH) to detect the SRY gene was used as control. RESULTS: Fifty 
      metaphases were scored. PRINS labeling showed signals for the SRY on the Y 
      chromosome at band Yp11.3 in all metaphases. These signals were as distinct as 
      that from results of FISH. CONCLUSION: This improved method is ideal for rapidly 
      localizing single copy genes and small DNA segments. And PRINS is a cost- and 
      time-effective alternative to FISH.
FAU - Nie, Yong
AU  - Nie Y
AD  - Institute of Medical Genetics, Sichuan University School of Life Science, Key 
      Laboratory of Bio-resources and Eco-environment, Ministry of Education, Chengdu, 
      Sichuan 610064, P. R. China.
FAU - Ding, Xianping
AU  - Ding X
FAU - Deng, Li
AU  - Deng L
FAU - Wei, Ping
AU  - Wei P
FAU - Wang, Huan
AU  - Wang H
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
SB  - IM
MH  - Gene Dosage
MH  - Genes, sry/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Metaphase/genetics
MH  - Primed In Situ Labeling/*methods
EDAT- 2008/12/10 09:00
MHDA- 2009/03/26 09:00
CRDT- 2008/12/10 09:00
PHST- 2008/12/10 09:00 [pubmed]
PHST- 2009/03/26 09:00 [medline]
PHST- 2008/12/10 09:00 [entrez]
AID - 940625153 [pii]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2008 Dec;25(6):701-3.