PMID- 19068099
OWN - NLM
STAT- MEDLINE
DCOM- 20090515
LR  - 20240109
IS  - 1462-5822 (Electronic)
IS  - 1462-5814 (Print)
IS  - 1462-5814 (Linking)
VI  - 11
IP  - 3
DP  - 2009 Mar
TI  - Type II fatty acid synthesis is essential only for malaria parasite late liver 
      stage development.
PG  - 506-20
LID - 10.1111/j.1462-5822.2008.01270.x [doi]
AB  - Intracellular malaria parasites require lipids for growth and replication. They 
      possess a prokaryotic type II fatty acid synthesis (FAS II) pathway that 
      localizes to the apicoplast plastid organelle and is assumed to be necessary for 
      pathogenic blood stage replication. However, the importance of FAS II throughout 
      the complex parasite life cycle remains unknown. We show in a rodent malaria 
      model that FAS II enzymes localize to the sporozoite and liver stage apicoplast. 
      Targeted deletion of FabB/F, a critical enzyme in fatty acid synthesis, did not 
      affect parasite blood stage replication, mosquito stage development and initial 
      infection in the liver. This was confirmed by knockout of FabZ, another critical 
      FAS II enzyme. However, FAS II-deficient Plasmodium yoelii liver stages failed to 
      form exo-erythrocytic merozoites, the invasive stage that first initiates blood 
      stage infection. Furthermore, deletion of FabI in the human malaria parasite 
      Plasmodium falciparum did not show a reduction in asexual blood stage replication 
      in vitro. Malaria parasites therefore depend on the intrinsic FAS II pathway only 
      at one specific life cycle transition point, from liver to blood.
FAU - Vaughan, Ashley M
AU  - Vaughan AM
AD  - Seattle Biomedical Research Institute, Seattle, WA 98109, USA.
FAU - O'Neill, Matthew T
AU  - O'Neill MT
FAU - Tarun, Alice S
AU  - Tarun AS
FAU - Camargo, Nelly
AU  - Camargo N
FAU - Phuong, Thuan M
AU  - Phuong TM
FAU - Aly, Ahmed S I
AU  - Aly AS
FAU - Cowman, Alan F
AU  - Cowman AF
FAU - Kappe, Stefan H I
AU  - Kappe SH
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20081203
PL  - India
TA  - Cell Microbiol
JT  - Cellular microbiology
JID - 100883691
RN  - 0 (Enzymes)
RN  - 0 (Fatty Acids)
RN  - 0 (Protozoan Proteins)
SB  - IM
MH  - Animals
MH  - Blood/*parasitology
MH  - Enzymes/genetics/metabolism
MH  - Fatty Acids/*biosynthesis
MH  - Female
MH  - Gene Deletion
MH  - Gene Knockout Techniques
MH  - Humans
MH  - Liver/*parasitology
MH  - Malaria/parasitology
MH  - Metabolic Networks and Pathways
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Organelles/chemistry
MH  - Plasmodium falciparum/chemistry/*growth & development/*metabolism
MH  - Plasmodium yoelii/chemistry/*growth & development/*metabolism
MH  - Protozoan Proteins/genetics/metabolism
PMC - PMC2688669
EDAT- 2008/12/11 09:00
MHDA- 2009/05/16 09:00
PMCR- 2009/06/01
CRDT- 2008/12/11 09:00
PHST- 2008/12/11 09:00 [pubmed]
PHST- 2009/05/16 09:00 [medline]
PHST- 2008/12/11 09:00 [entrez]
PHST- 2009/06/01 00:00 [pmc-release]
AID - CMI1270 [pii]
AID - 10.1111/j.1462-5822.2008.01270.x [doi]
PST - ppublish
SO  - Cell Microbiol. 2009 Mar;11(3):506-20. doi: 10.1111/j.1462-5822.2008.01270.x. 
      Epub 2008 Dec 3.