PMID- 19068130
OWN - NLM
STAT- MEDLINE
DCOM- 20090319
LR  - 20240316
IS  - 1472-6882 (Electronic)
IS  - 1472-6882 (Linking)
VI  - 8
DP  - 2008 Dec 9
TI  - Antioxidant and free radical scavenging activity of Spondias pinnata.
PG  - 63
LID - 10.1186/1472-6882-8-63 [doi]
AB  - BACKGROUND: Many diseases are associated with oxidative stress caused by free 
      radicals. Current research is directed towards finding naturally-occurring 
      antioxidants of plant origin. The aim of the present study was to evaluate the in 
      vitro antioxidant activities of Spondias pinnata stem bark extract. METHODS: A 
      70% methanol extract of Spondias pinnata stem bark was studied in vitro for total 
      antioxidant activity, for scavenging of hydroxyl radicals, superoxide anions, 
      nitric oxide, hydrogen peroxide, peroxynitrite, singlet oxygen and hypochlorous 
      acid, and for iron chelating capacity, reducing power, and phenolic and flavonoid 
      contents. RESULTS: The extract showed total antioxidant activity with a trolox 
      equivalent antioxidant concentration (TEAC) value of 0.78 +/- 0.02. The IC50 
      values for scavenging of free radicals were 112.18 +/- 3.27 microg/ml, 13.46 +/- 
      0.66 microg/ml and 24.48 +/- 2.31 microg/ml for hydroxyl, superoxide and nitric 
      oxide, respectively. The IC50 for hydrogen peroxide scavenging was 44.74 +/- 
      25.61 mg/ml. For the peroxynitrite, singlet oxygen and hypochlorous acid 
      scavenging activities the IC50 values were 716.32 +/- 32.25 microg/ml, 58.07 +/- 
      5.36 microg/ml and 127.99 +/- 6.26 microg/ml, respectively. The extract was found 
      to be a potent iron chelator with IC50 = 66.54 +/- 0.84 microg/ml. The reducing 
      power was increased with increasing amounts of extract. The plant extract (100 
      mg) yielded 91.47 +/- 0.004 mg/ml gallic acid-equivalent phenolic content and 
      350.5 +/- 0.004 mg/ml quercetin-equivalent flavonoid content. CONCLUSION: The 
      present study provides evidence that a 70% methanol extract of Spondias pinnata 
      stem bark is a potential source of natural antioxidants.
FAU - Hazra, Bibhabasu
AU  - Hazra B
AD  - Division of Molecular Medicine, Bose Institute, P-1/12 CIT Scheme VIIM, 
      Kolkata-700054, India. bibhabasuhazra@yahoo.com
FAU - Biswas, Santanu
AU  - Biswas S
FAU - Mandal, Nripendranath
AU  - Mandal N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081209
PL  - England
TA  - BMC Complement Altern Med
JT  - BMC complementary and alternative medicine
JID - 101088661
RN  - 0 (Antioxidants)
RN  - 0 (Chelating Agents)
RN  - 0 (Flavonoids)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Free Radicals)
RN  - 0 (Iron Chelating Agents)
RN  - 0 (Phenols)
RN  - 0 (Plant Extracts)
RN  - E1UOL152H7 (Iron)
SB  - IM
MH  - Anacardiaceae/*chemistry
MH  - Antioxidants/isolation & purification/*pharmacology
MH  - Chelating Agents/isolation & purification/*pharmacology
MH  - Flavonoids/isolation & purification
MH  - Free Radical Scavengers/isolation & purification/*pharmacology
MH  - Free Radicals/*metabolism
MH  - Iron/metabolism
MH  - Iron Chelating Agents/isolation & purification/pharmacology
MH  - Oxidative Stress/*drug effects
MH  - Phenols/isolation & purification
MH  - Plant Bark/chemistry
MH  - Plant Extracts/chemistry/isolation & purification/*pharmacology
MH  - Plant Stems/chemistry
PMC - PMC2636748
EDAT- 2008/12/11 09:00
MHDA- 2009/03/20 09:00
PMCR- 2008/12/09
CRDT- 2008/12/11 09:00
PHST- 2008/06/30 00:00 [received]
PHST- 2008/12/09 00:00 [accepted]
PHST- 2008/12/11 09:00 [pubmed]
PHST- 2009/03/20 09:00 [medline]
PHST- 2008/12/11 09:00 [entrez]
PHST- 2008/12/09 00:00 [pmc-release]
AID - 1472-6882-8-63 [pii]
AID - 10.1186/1472-6882-8-63 [doi]
PST - epublish
SO  - BMC Complement Altern Med. 2008 Dec 9;8:63. doi: 10.1186/1472-6882-8-63.