PMID- 1906872
OWN - NLM
STAT- MEDLINE
DCOM- 19910829
LR  - 20190508
IS  - 0021-9193 (Print)
IS  - 1098-5530 (Electronic)
IS  - 0021-9193 (Linking)
VI  - 173
IP  - 15
DP  - 1991 Aug
TI  - Replacement recombination in Lactococcus lactis.
PG  - 4794-8
AB  - In the pUC18-derived integration plasmid pML336 there is a 5.3-kb chromosomal DNA 
      fragment that carries the X-prolyl dipeptidyl aminopeptidase gene (pepXP). The 
      gene was inactivated by the insertion of an erythromycin resistance determinant 
      into its coding sequence. Covalently closed circular DNA of pML336 was used for 
      the electrotransformation of Lactococcus lactis. In 2% of the 
      erythromycin-resistant transformants the pepXP gene was inactivated by a 
      double-crossover event (replacement recombination) between pML336 and the L. 
      lactis chromosome. The other transformants in which the pepXP gene had not been 
      inactivated carried a Campbell-type integrated copy of the plasmid. Loss of part 
      of the Campbell-type integrated plasmid via recombination between 1.6-kb 
      nontandem repeats occurred with low frequencies that varied between less than 2.8 
      x 10(-6) and 8.5 x 10(-6), producing cells with a chromosomal structure like that 
      of cells in which replacement recombination had taken place.
FAU - Leenhouts, K J
AU  - Leenhouts KJ
AD  - Institute of Genetics, University of Groningen, The Netherlands.
FAU - Kok, J
AU  - Kok J
FAU - Venema, G
AU  - Venema G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Bacteriol
JT  - Journal of bacteriology
JID - 2985120R
SB  - IM
GS  - pepXP
MH  - Chromosomes, Bacterial
MH  - Crossing Over, Genetic
MH  - Gene Expression Regulation, Bacterial
MH  - Genes, Bacterial
MH  - Lactococcus lactis/drug effects/enzymology/*genetics
MH  - Molecular Weight
MH  - Plasmids
MH  - *Recombination, Genetic
MH  - Repetitive Sequences, Nucleic Acid
PMC - PMC208158
EDAT- 1991/08/01 00:00
MHDA- 1991/08/01 00:01
PMCR- 1991/08/01
CRDT- 1991/08/01 00:00
PHST- 1991/08/01 00:00 [pubmed]
PHST- 1991/08/01 00:01 [medline]
PHST- 1991/08/01 00:00 [entrez]
PHST- 1991/08/01 00:00 [pmc-release]
AID - 10.1128/jb.173.15.4794-4798.1991 [doi]
PST - ppublish
SO  - J Bacteriol. 1991 Aug;173(15):4794-8. doi: 10.1128/jb.173.15.4794-4798.1991.