PMID- 19070120
OWN - NLM
STAT- MEDLINE
DCOM- 20090105
LR  - 20190907
IS  - 1028-8880 (Print)
IS  - 1028-8880 (Linking)
VI  - 10
IP  - 15
DP  - 2007 Aug 1
TI  - Assessment of genetic diversity of local and exotic Brassica napus germplasm.
PG  - 2490-4
AB  - Estimation of genetic diversity of Brassica germplasm provides the basis for 
      rapeseed/mustard genetic improvement. Studies were undertaken to estimate the 
      genetic diversity of 30 lines of Brassica napus using Randomly Amplified 
      Polymorphic DNA (RAPD) primers. A total of 30 B. napus genotypes of local and 
      exotic origin were characterized using molecular markers. Four RAPD primers were 
      used to estimate the genetic distances among the genotypes in all the possible 
      combinations. The genetic diversity study revealed different levels of genetic 
      polymorphism for RAPD primers GLA05, GLA07, GLA09 and GLA 10, resulting in 
      amplification of 5.7, 3.5, 3.1 and 5.4 scorable bands (loci) per genotype, 
      respectively. Individual genetic distances observed among B. napus genotypes 
      ranged from 6.5 to 51%. Bivariate data matrix was generated and genetic distances 
      were calculated using Unweighted Pair Group of Arithmetic Mean (UPGMA) procedure. 
      The UPGMA cluster analyses revealed maximum genetic dissimilarity for 8966-1 and 
      8969-1 genotypes, closely followed by Ganyou-5, 89127-1, 89111-2 and Mlep-048. It 
      is recommended that among the thirty B. napus genotypes, genetically distinct 
      lines pointed out in the present study, should be used in future breeding 
      programs for improvement of Brassica napus.
FAU - Ahmad, Mian Afaq
AU  - Ahmad MA
AD  - Institute of Biotechnology and Genetic Engineering, NWFP Agricultural University, 
      Peshawar, Pakistan.
FAU - Munir, Iqbal
AU  - Munir I
FAU - Ali, Waqar
AU  - Ali W
FAU - Swati, Zahoor Ahmad
AU  - Swati ZA
FAU - Khattak, Muhammad Saeed
AU  - Khattak MS
FAU - Sohail, Quahir
AU  - Sohail Q
FAU - Khan, Imran
AU  - Khan I
LA  - eng
PT  - Journal Article
PL  - Pakistan
TA  - Pak J Biol Sci
JT  - Pakistan journal of biological sciences : PJBS
JID - 101247723
SB  - IM
MH  - Brassica napus/classification/*genetics
MH  - *Genetic Variation
MH  - Genotype
MH  - Phylogeny
MH  - Random Amplified Polymorphic DNA Technique/methods
EDAT- 2007/08/01 00:00
MHDA- 2009/01/06 09:00
CRDT- 2008/12/17 09:00
PHST- 2008/12/17 09:00 [entrez]
PHST- 2007/08/01 00:00 [pubmed]
PHST- 2009/01/06 09:00 [medline]
AID - 10.3923/pjbs.2007.2490.2494 [doi]
PST - ppublish
SO  - Pak J Biol Sci. 2007 Aug 1;10(15):2490-4. doi: 10.3923/pjbs.2007.2490.2494.