PMID- 19070360
OWN - NLM
STAT- MEDLINE
DCOM- 20090305
LR  - 20220318
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 30
IP  - 7
DP  - 2009 Mar
TI  - Engineered skeletal muscle tissue networks with controllable architecture.
PG  - 1401-12
LID - 10.1016/j.biomaterials.2008.11.015 [doi]
AB  - The engineering of functional skeletal muscle tissue substitutes holds promise 
      for the treatment of various muscular diseases and injuries. However, no tissue 
      fabrication technology currently exists for the generation of a relatively large 
      and thick bioartificial muscle made of densely packed, uniformly aligned, and 
      differentiated myofibers. In this study, we describe a versatile cell/hydrogel 
      micromolding approach where polydimethylsiloxane (PDMS) molds containing an array 
      of elongated posts were used to fabricate relatively large neonatal rat skeletal 
      muscle tissue networks with reproducible and controllable architecture. By 
      combining cell-mediated fibrin gel compaction and precise microfabrication of 
      mold dimensions including the length and height of the PDMS posts, we were able 
      to simultaneously support high cell viability, guide cell alignment along the 
      microfabricated tissue pores, and reproducibly control the overall tissue 
      porosity, size, and thickness. The interconnected muscle bundles within the 
      porous tissue networks were composed of densely packed, aligned, and highly 
      differentiated myofibers. The formed myofibers expressed myogenin, developed 
      abundant cross-striations, and generated spontaneous tissue contractions at the 
      macroscopic spatial scale. The proliferation of non-muscle cells was 
      significantly reduced compared to monolayer cultures. The more complex muscle 
      tissue architectures were fabricated by controlling the spatial distribution and 
      direction of the PDMS posts.
FAU - Bian, Weining
AU  - Bian W
AD  - Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
FAU - Bursac, Nenad
AU  - Bursac N
LA  - eng
GR  - AR055226/AR/NIAMS NIH HHS/United States
GR  - HL080469/HL/NHLBI NIH HHS/United States
GR  - R21 HL080469-02/HL/NHLBI NIH HHS/United States
GR  - R01 AR055226-01A1/AR/NIAMS NIH HHS/United States
GR  - R21 HL080469/HL/NHLBI NIH HHS/United States
GR  - R01 AR055226/AR/NIAMS NIH HHS/United States
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20081212
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Biocompatible Materials)
RN  - 0 (Hydrogels)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials/chemistry/metabolism
MH  - Cell Differentiation
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Hydrogels/chemistry/metabolism
MH  - Materials Testing
MH  - Mice
MH  - Muscle, Skeletal/*cytology/physiology
MH  - Porosity
MH  - Rats
MH  - Surface Properties
MH  - *Tissue Engineering/instrumentation/methods
MH  - *Tissue Scaffolds
PMC - PMC2726993
MID - NIHMS92412
EDAT- 2008/12/17 09:00
MHDA- 2009/03/06 09:00
PMCR- 2010/03/01
CRDT- 2008/12/17 09:00
PHST- 2008/08/18 00:00 [received]
PHST- 2008/11/09 00:00 [accepted]
PHST- 2008/12/17 09:00 [entrez]
PHST- 2008/12/17 09:00 [pubmed]
PHST- 2009/03/06 09:00 [medline]
PHST- 2010/03/01 00:00 [pmc-release]
AID - S0142-9612(08)00878-8 [pii]
AID - 10.1016/j.biomaterials.2008.11.015 [doi]
PST - ppublish
SO  - Biomaterials. 2009 Mar;30(7):1401-12. doi: 10.1016/j.biomaterials.2008.11.015. 
      Epub 2008 Dec 12.