PMID- 19071351
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20081216
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 71
IP  - 2
DP  - 2007 Feb 15
TI  - Development of an extraction and cleanup procedure for a liquid 
      chromatographic-mass spectrometric method to analyze 
      octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine in eggs.
PG  - 627-31
LID - 10.1016/j.talanta.2006.05.007 [doi]
AB  - An efficient sample extraction and cleanup method was developed for determination 
      of octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX) in eggs. The procedure 
      included solvent extraction of HMX from eggs followed by cleanup using florisil 
      and styrene-divinylbenzene (SDB) cartridges. Homogenized egg aliquots were 
      thoroughly mixed with 10mL acetonitrile and extracted with ultrasonication for 
      1h. Each sample was centrifuged and all liquid was collected for cleanup. After 
      concentration by N(2) evaporation, each extract was cleaned by florisil and SDB 
      cartridges to remove endogenous interfering compounds. Finally, each extract was 
      filtered through a 0.2microm PTFE membrane and stored for liquid 
      chromatographic-mass spectrometric (LC-MS) analysis. Chromatographic separation 
      was achieved on a reverse phase (RP) C18 column, with a mobile phase containing 
      60% methanol+40% 1.0mM acetic acid aqueous solution. Acetic acid was employed as 
      mobile phase additive to form negatively charged adduct ions [M+CH(3)COO](-), and 
      m/z=355 was quantified by selective ion monitoring (SIM). Overall recoveries from 
      eggs containing 10, 50, 250 and 1000ng/g of HMX were 84.0%, 88.0%, 90.6% and 
      87.4%. A method detection limit (MDL) of 0.15ng/g was achieved.
FAU - Liu, Jun
AU  - Liu J
AD  - The Institute of Environmental and Human Health, Department of Environmental 
      Toxicology, Texas Tech University, Lubbock, TX 79409-1163, USA. 
      jun.liu@tiehh.ttu.edu
FAU - Severt, Scott A
AU  - Severt SA
FAU - Pan, Xiaoping
AU  - Pan X
FAU - Smith, Philip N
AU  - Smith PN
FAU - McMurry, Scott T
AU  - McMurry ST
FAU - Cobb, George P
AU  - Cobb GP
LA  - eng
PT  - Journal Article
DEP - 20060621
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
EDAT- 2007/02/15 00:00
MHDA- 2007/02/15 00:01
CRDT- 2008/12/17 09:00
PHST- 2006/03/28 00:00 [received]
PHST- 2006/05/05 00:00 [revised]
PHST- 2006/05/05 00:00 [accepted]
PHST- 2008/12/17 09:00 [entrez]
PHST- 2007/02/15 00:00 [pubmed]
PHST- 2007/02/15 00:01 [medline]
AID - S0039-9140(06)00319-5 [pii]
AID - 10.1016/j.talanta.2006.05.007 [doi]
PST - ppublish
SO  - Talanta. 2007 Feb 15;71(2):627-31. doi: 10.1016/j.talanta.2006.05.007. Epub 2006 
      Jun 21.