PMID- 19077460
OWN - NLM
STAT- MEDLINE
DCOM- 20090717
LR  - 20181201
IS  - 1424-3911 (Electronic)
IS  - 1424-3903 (Linking)
VI  - 9
IP  - 1-2
DP  - 2009
TI  - Pancreatitis-associated protein inhibits human pancreatic stellate cell MMP-1 and 
      -2, TIMP-1 and -2 secretion and RECK expression.
PG  - 99-110
LID - 10.1159/000178880 [doi]
AB  - BACKGROUND/AIMS: Pancreatic stellate cells (PSCs) play a key role in fibrogenesis 
      associated with acute and chronic pancreatitis. Pancreatitis-associated protein 
      (PAP), an acute-phase protein, is dramatically upregulated during acute and 
      chronic pancreatitis. Assuming a protective role of PAP, we investigated its 
      effects on human PSCs. METHODS: PSCs were obtained by outgrowth from fibrotic 
      human pancreas tissue. PAP was expressed in the yeast Pichia pastoris. PAP was 
      added at 10 ng/ml to cultured PSCs. Cell proliferation was determined by 
      bromodeoxyuridine incorporation. PSC migration was assessed by a wound healing 
      assay. Collagen types I and III, fibronectin, matrix metalloproteinases (MMPs), 
      tissue inhibitors of MMPs (TIMPs) and reversion-inducing cysteine-rich protein 
      with Kazal motifs (RECK) were demonstrated on protein and mRNA level. RESULTS: 
      PAP had no significant effect on PSC proliferation and migration. Cell-associated 
      fibrillar collagen types I and III and fibronectin increased after addition of 
      PAP to PSCs. PAP diminished the expression of MMP-1 and -2 and TIMP-1 and -2 and 
      their concentrations in PSC supernatants. RECK was detected on the surface of 
      PSCs and its expression was reduced after PAP application. CONCLUSIONS: Our data 
      offer new insights into the biological functions of PAP, which may play an 
      important role in wound healing response and cell-matrix interactions.
CI  - Copyright 2008 S. Karger AG, Basel and IAP.
FAU - Li, Ling
AU  - Li L
AD  - Department of Clinical Chemistry, University Hospital Ulm, Ulm, Germany.
FAU - Bachem, Max G
AU  - Bachem MG
FAU - Zhou, Shaoxia
AU  - Zhou S
FAU - Sun, Zilin
AU  - Sun Z
FAU - Chen, Jinfei
AU  - Chen J
FAU - Siech, Marco
AU  - Siech M
FAU - Bimmler, Daniel
AU  - Bimmler D
FAU - Graf, Rolf
AU  - Graf R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081212
PL  - Switzerland
TA  - Pancreatology
JT  - Pancreatology : official journal of the International Association of 
      Pancreatology (IAP) ... [et al.]
JID - 100966936
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (GPI-Linked Proteins)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Pancreatitis-Associated Proteins)
RN  - 0 (RECK protein, human)
RN  - 0 (REG3A protein, human)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Antigens, Neoplasm/*physiology
MH  - Biomarkers, Tumor/*physiology
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - GPI-Linked Proteins
MH  - Gene Expression/drug effects
MH  - Humans
MH  - Lectins, C-Type/*physiology
MH  - Matrix Metalloproteinase 1/metabolism
MH  - Matrix Metalloproteinase 2/metabolism
MH  - *Matrix Metalloproteinase Inhibitors
MH  - Membrane Glycoproteins/*biosynthesis
MH  - Pancreas/cytology/*drug effects
MH  - Pancreatitis-Associated Proteins
MH  - Tissue Inhibitor of Metalloproteinase-1/*antagonists & inhibitors/metabolism
MH  - Tissue Inhibitor of Metalloproteinase-2/*antagonists & inhibitors/metabolism
EDAT- 2008/12/17 09:00
MHDA- 2009/07/18 09:00
CRDT- 2008/12/17 09:00
PHST- 2007/10/21 00:00 [received]
PHST- 2008/03/21 00:00 [accepted]
PHST- 2008/12/17 09:00 [entrez]
PHST- 2008/12/17 09:00 [pubmed]
PHST- 2009/07/18 09:00 [medline]
AID - S1424-3903(09)80075-6 [pii]
AID - 10.1159/000178880 [doi]
PST - ppublish
SO  - Pancreatology. 2009;9(1-2):99-110. doi: 10.1159/000178880. Epub 2008 Dec 12.