PMID- 19078169
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130704
LR  - 20191111
IS  - 1076-1608 (Print)
IS  - 1076-1608 (Linking)
VI  - 3
IP  - 3
DP  - 1997 Jun
TI  - Molecular biology and immunology for clinicians DNA polymerase and the polymerase 
      chain reaction.
PG  - 135-9
AB  - By using enzymes that underlie the molecular mechanisms of normal cell function, 
      scientists have advanced the molecular biology of research and diagnostic 
      testing. Normal cells divide and in so doing must accurately replicate their DNA; 
      one of the enzymes crucial in making exact copies of DNA is DNA polymerase, which 
      is at the heart of the polymerase chain reaction. This technique allows one to 
      make billions or trillions of copies from a single molecule of DNA in a few 
      hours, levels of DNA easily detectable by techniques described earlier in this 
      series. The polymerase chain reaction can be used for clinical testing, e.g., 
      identification of DNA derived from a micro-organism. Also, one can clone DNA in 
      large quantities and then determine the specific nucleotide sequences. This then 
      allows one to study the DNA of certain proteins in individuals with a specific 
      disease process and how these DNA sequences differ from those in unaffected 
      people. With this new technology, we can identify the following: variant 
      collagens that underlie familial osteoarthritis; the presence of the DNA of 
      micro-organisms, such as Ureaplasma and Chlamydia, at the site of inflammatory 
      joint diseases, establishing the infectious nature of the synovitis; different 
      human leukocyte antigen (HLA)-B27 alleles that predispose patients to the 
      development of the seronegative spondylarthropathies; and characteristics of 
      different HLA class II molecules that may yield insights into antigen 
      presentation and its role in the pathogenesis of rheumatoid arthritis.
FAU - Sigal, L H
AU  - Sigal LH
AD  - Department of Medicine, Department of Pediatrics, Department of Molecular 
      Genetics and Microbiology, Lyme Disease Center; Division of Rheumatology and 
      Connective. Tissue Research, University of Medicine and Dentistry of New 
      Jersey-Robert Wood Johnson Medical School, Brunswick. New Jersey.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Clin Rheumatol
JT  - Journal of clinical rheumatology : practical reports on rheumatic & 
      musculoskeletal diseases
JID - 9518034
EDAT- 1997/06/01 00:00
MHDA- 1997/06/01 00:01
CRDT- 2008/12/17 09:00
PHST- 2008/12/17 09:00 [entrez]
PHST- 1997/06/01 00:00 [pubmed]
PHST- 1997/06/01 00:01 [medline]
AID - 00124743-199706000-00003 [pii]
AID - 10.1097/00124743-199706000-00003 [doi]
PST - ppublish
SO  - J Clin Rheumatol. 1997 Jun;3(3):135-9. doi: 10.1097/00124743-199706000-00003.