PMID- 1908039
OWN - NLM
STAT- MEDLINE
DCOM- 19910917
LR  - 20190820
IS  - 0385-5600 (Print)
IS  - 0385-5600 (Linking)
VI  - 35
IP  - 1
DP  - 1991
TI  - Demonstration and characterization of the cell wall carbohydrate and protein 
      antigens from Clostridium botulinum type E Saroma.
PG  - 27-37
AB  - Two different cell wall antigens, carbohydrate (CHO) and protein (P), from 
      Clostridium botulinum type E Saroma were extracted with sodium dodecyl sulfate 
      (SDS) and purified by chromatography on DEAE-Sepharose CL-6B and Sephadex G-75 or 
      G-100. The CHO antigen was composed of glucose, galactose, glucosamine, 
      galactosamine, alanine and phosphorus with a molar ratio of 
      1.5:1.5:0.25:0.25:1:1. The P antigen was an acidic protein with a molecular 
      weight of 60 kDa, in which the major amino acids were aspartate, glutamate and 
      serine, while the minor ones were cysteine and methionine. Thin sections of the 
      intact or SDS-extracted cells of the organism demonstrated that the cell wall was 
      composed of a two-layered structure, an inner layer about 20 nm thick and an 
      outer layer about 10 nm, and by the extraction with SDS, the outer layer 
      disappeared from the cell surface, leaving the inner layer. Immunogel diffusion 
      tests demonstrated that either CHO antigen or P antigen was common among the 
      nonproteolytic strains of C. botulinum.
FAU - Takumi, K
AU  - Takumi K
AD  - Department of Food Microbiology, Tokushima University School of Medicine.
FAU - Nagao, S
AU  - Nagao S
FAU - Takeoka, A
AU  - Takeoka A
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - Microbiol Immunol
JT  - Microbiology and immunology
JID - 7703966
RN  - 0 (Amino Acids)
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Carbohydrates)
SB  - IM
MH  - Amino Acids/analysis
MH  - Antigens, Bacterial/chemistry/*isolation & purification
MH  - Carbohydrates/chemistry/*isolation & purification
MH  - Cell Wall/*immunology
MH  - Chromatography, Gel
MH  - Clostridium botulinum/*immunology
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Immunodiffusion
MH  - Immunoelectrophoresis, Two-Dimensional
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
AID - 10.1111/j.1348-0421.1991.tb01530.x [doi]
PST - ppublish
SO  - Microbiol Immunol. 1991;35(1):27-37. doi: 10.1111/j.1348-0421.1991.tb01530.x.