PMID- 19080644
OWN - NLM
STAT- MEDLINE
DCOM- 20090528
LR  - 20221207
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 88
IP  - 36
DP  - 2008 Sep 23
TI  - [Fluorescent in situ hybridization with a panel of probes detects molecular 
      cytogenetic abnormalities in patients with chronic lymphocytic leukemia].
PG  - 2537-40
AB  - OBJECTIVE: To explore the characteristics and prognostic significance of 
      molecular cytogenetic aberrations in Chinese patients with chronic lymphocytic 
      leukemia (CLL) and the significance thereof in diagnosis of CLL. METHODS: A panel 
      of probes (LSI D13S319, LSI p53, LSI ATM, CEP 12, LSI MYB, and LSI IGHC/IGHV) and 
      interphase fluorescence in situ hybridization (FISH) were used to prospectively 
      detect the cytogenetic abnormalities in 106 CLL patients, 82 males and 24 
      females, aged 62 (34 - 86). RESULTS: Molecular cytogenetic aberrations were found 
      in 79 of the 106 CLL patients (74.5%) and 35 patients (33.0%) showed more than 
      two kinds of abnormalities. The most frequent abnormality detected was del 
      (13q14) in 48 cases (45.3%), followed by trisomy of chromosome 12 in 27 patients 
      (25.5%), IgH translocation in 25 patients (23.6%), del (17p13) in 17 patients 
      (16.0%), del (11q22) in 11 patients (10.5%) and del (6q23) in 5 patients (4.7%). 
      The Del (13q14) rate of the group younger than 60 was 56.5%, significantly higher 
      than that of the group aged > or = 60 (36.7%, P = 0.033). There was no 
      significant relationship between molecular cytogenetic aberrations and sex and 
      Binet stages (both P > 0.05). Kaplan-Meier survival analysis showed that the 
      survival time was shorter in the patients with p53 or ATM gene deletion. Patients 
      with sole Del (13q14) had longer survival time than those with other 
      abnormalities. CONCLUSION: The frequencies of the chromosomal abnormalities in 
      Chinese CLL patients are similar to those in Western countries. Panel FISH has 
      greatly increased the sensitivity of cytogenetic analyses. Del (13q14) is the 
      most frequent abnormality in CLL. Molecular cytogenetic aberrations detected with 
      FISH have important prognostic significance in CLL.
FAU - Xu, Wei
AU  - Xu W
AD  - Department of Hematology, First Affiliated Hospital of Nanjing Medical 
      University, Jiangsu Province Hospital, Nanjing 210029, China.
FAU - Li, Jian-yong
AU  - Li JY
FAU - Li, Li
AU  - Li L
FAU - Yu, Hui
AU  - Yu H
FAU - Shen, Qiu-dan
AU  - Shen QD
FAU - Fan, Lei
AU  - Fan L
FAU - Qiao, Chun
AU  - Qiao C
FAU - Hong, Ming
AU  - Hong M
FAU - Qian, Si-xuan
AU  - Qian SX
FAU - Qiu, Hong-xia
AU  - Qiu HX
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (Nucleic Acid Probes)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Asian People
MH  - *Chromosome Aberrations
MH  - Cytogenetic Analysis
MH  - Female
MH  - Follow-Up Studies
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/ethnology/*genetics/mortality
MH  - Male
MH  - Middle Aged
MH  - Nucleic Acid Probes
MH  - Survival Rate
EDAT- 2008/12/17 09:00
MHDA- 2009/05/29 09:00
CRDT- 2008/12/17 09:00
PHST- 2008/12/17 09:00 [entrez]
PHST- 2008/12/17 09:00 [pubmed]
PHST- 2009/05/29 09:00 [medline]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2008 Sep 23;88(36):2537-40.