PMID- 19080714
OWN - NLM
STAT- MEDLINE
DCOM- 20090612
LR  - 20210915
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 88
IP  - 37
DP  - 2008 Oct 14
TI  - [Effects of dihydroartiminisin on the adhesion, migration, and invasion of 
      epithelial ovarian cancer cells].
PG  - 2642-6
AB  - OBJECTIVE: To investigate the effects of dihydroartiminisin (DHA) on the 
      adhesion, migration, and invasion ovarian cancer cells. METHODS: Human ovarian 
      cancer cells of the lines SKOV3 and OVCAR3 were cultured. Suspensions of SKOV3 
      and OVCAR3 cells were treated with DHA of the concentrations of 0.5, 2.5, 12.5, 
      and 62.5 micromol/L respectively, and then inoculated on the plate coated with 
      Matrigel. MTT method was used to -determine the adhesion rate. Transwell membrane 
      chamber model was used to evaluate the effect of DHA on the migration and 
      invasion of the SKOV3 and OVCAR3 cells. Western blotting and reverse 
      transcriptase polymerase chain reaction were used to detect the effect of DHA on 
      the phosphorylation of focal adhesion kinase (FAK) and on the effect of 
      expression of metal matrix proteinases (MMPs) and their tissue inhibitors (TIMPs) 
      respectively. RESULTS: (1) Compared to the cells without DHA treatment, the cell 
      adhesion ability levels of the SKOV3 and OVCAR3 cells treated with 12.5 
      micromol/L DHA decreased by 76.1% and 57.9% respectively (P < 0.05), while their 
      migration ability levels decreased by 59.3% and 69.7% respectively (P < 0.05). 
      (2) Both SKOV3 and OVCAR3 showed weak invasion ability, and DHA only showed a 
      slight inhibitory effect on the cell invasion of these 2 lines (both P > 0.05). 
      (3) Compared to the cells without DHA treatment, the phosphorylation level of FAK 
      of the SKOV3 and OVCAR3 cells treated with 12.5 micromol/L DHA decreased by 42.9% 
      and 44.8% respectively (both P < 0.05). (4) RT-PCR showed mRNA expression of 
      MMP2, TIMP1, and TIMP2, but not mRNA expression of MMP9 in both SKOV3 and OVCAR3 
      cells. The mRNA expression levels of the SKOV3 and OVCAR3 cells treated with 12.5 
      micromol/L DHA increased by 1.5 and 2.6 times respectively (both P < 0.05). 
      CONCLUSION: DHA has inhibitory effects on the adhesion and migration of 
      epithelial ovarian cancer cells, which may be related to its down-regulation of 
      the phosphorylation of FAK in these cells.
FAU - Tan, Xian-Jie
AU  - Tan XJ
AD  - Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, 
      Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 
      100730, China.
FAU - Lang, Jing-He
AU  - Lang JH
FAU - Plouet, Jean
AU  - Plouet J
FAU - Wu, Ming
AU  - Wu M
FAU - Shen, Keng
AU  - Shen K
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (Artemisinins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - 6A9O50735X (artenimol)
RN  - EC 2.7.10.2 (Focal Adhesion Protein-Tyrosine Kinases)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Apoptosis
MH  - Artemisinins/*pharmacology
MH  - Cell Adhesion/*drug effects
MH  - Cell Line, Tumor
MH  - Cell Movement/*drug effects
MH  - Down-Regulation
MH  - Female
MH  - Focal Adhesion Protein-Tyrosine Kinases/genetics
MH  - Humans
MH  - Matrix Metalloproteinases/genetics
MH  - Neoplasm Invasiveness
MH  - Ovarian Neoplasms/*metabolism/*pathology
MH  - Phosphorylation
MH  - RNA, Messenger/genetics
MH  - Tissue Inhibitor of Metalloproteinases/genetics
EDAT- 2008/12/17 09:00
MHDA- 2009/06/13 09:00
CRDT- 2008/12/17 09:00
PHST- 2008/12/17 09:00 [entrez]
PHST- 2008/12/17 09:00 [pubmed]
PHST- 2009/06/13 09:00 [medline]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2008 Oct 14;88(37):2642-6.