PMID- 19086383 OWN - NLM STAT- MEDLINE DCOM- 20090423 LR - 20191111 IS - 0034-7744 (Print) IS - 0034-7744 (Linking) VI - 55 IP - 3-4 DP - 2007 Sep-Dec TI - Diversity and effectiveness of tropical mangrove soil microflora on the degradation of polythene carry bags. PG - 777-86 AB - The diversity and load of heterotrophic bacteria and fungi associated with the mangrove soil from Suva, Fiji Islands, was determined by using the plate count method. The ability of the bacterial isolates to produce various hydrolytic enzymes such as amylase, gelatinase and lipase were determined using the plate assay. The heterotrophic bacterial load was considerably higher than the fungal load. There was a predominance of the gram positive genus, Bacillus. Other genera encountered included Staphylococcus, Micrococcus, Listeria and Vibrio. Their effectiveness on the degradation of commercial polythene carry bags made of high density polyethylene (HDPE) and low density polyethylene (LDPE) was studied over a period of eight weeks in the laboratory. Biodegradation was measured in terms of mean weight loss, which was nearly 5 % after a period of eight weeks. There was a significant increase in the bacterial load of the soil attached to class 2 (HDPE) polythene. After eight weeks of submergence in mangrove soil, soil attached to class 1 and class 3 polythene mostly had Bacillus (Staphylococcus predominated in class 2 polythene). While most of the isolates were capable of producing hydrolytic enzymes such as amylase and gelatinase, lipolytic activity was low. Class 2 HDPE suffered the greatest biodegradation. FAU - Kumar, Shristi AU - Kumar S AD - Department of Biology, School of Pure and Applied Sciences, The University of the South Pacific, P.O. Box, 1168, Suva, Fiji Islands. shristikumar@yahoo.com FAU - Hatha, A A M AU - Hatha AA FAU - Christi, K S AU - Christi KS LA - eng PT - Journal Article PL - Costa Rica TA - Rev Biol Trop JT - Revista de biologia tropical JID - 0404267 RN - 9002-88-4 (Polyethylene) RN - EC 3.- (Hydrolases) SB - IM MH - Biodegradation, Environmental MH - Biodiversity MH - Fungi/classification/enzymology/*metabolism MH - Gram-Negative Bacteria/classification/enzymology/*metabolism MH - Gram-Positive Bacteria/classification/enzymology/*metabolism MH - Hydrolases/*biosynthesis MH - Polyethylene/*metabolism MH - Rhizophoraceae MH - *Soil Microbiology MH - Time Factors EDAT- 2007/09/01 00:00 MHDA- 2009/04/25 09:00 CRDT- 2008/12/18 09:00 PHST- 2008/12/18 09:00 [entrez] PHST- 2007/09/01 00:00 [pubmed] PHST- 2009/04/25 09:00 [medline] AID - 10.15517/rbt.v55i3-4.5954 [doi] PST - ppublish SO - Rev Biol Trop. 2007 Sep-Dec;55(3-4):777-86. doi: 10.15517/rbt.v55i3-4.5954.