PMID- 19087271
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20100520
LR  - 20211020
IS  - 1746-1596 (Electronic)
IS  - 1746-1596 (Linking)
VI  - 3
DP  - 2008 Dec 16
TI  - LED-FISH: Fluorescence microscopy based on light emitting diodes for the 
      molecular analysis of Her-2/neu oncogene amplification.
PG  - 49
LID - 10.1186/1746-1596-3-49 [doi]
AB  - Light emitting diodes (LED), which are available as small monochromatic light 
      sources with characteristic features such as maximum illumination power combined 
      with minimum energy consumption and extremely long lifespan have already proved 
      as a highly potential low-cost alternative for specific diagnostic applications 
      in clinical medicine such as tuberculosis fluorescence microscopy. Likewise, the 
      most reliable evaluation of Her-2/neu (c-erbB2) gene amplification, which has 
      been established in the last few years for routine diagnosis in clinical 
      pathology as determinant towards Herceptin-based treatment of patients with 
      breast cancer, is based on fluorescence in situ hybridization (FISH) and 
      corresponding high priced fluorescence equipment. In order to test the 
      possibility to utilize the advantages of low-cost LED technology on FISH analysis 
      of c-erbB2 gene expression for routine diagnostic purposes, the applicability of 
      a standard bright field Carl Zeiss Axiostar Plus microscope equipped with a Fraen 
      AFTER (Amplified Fluorescence by Transmitted Excitation of Radiation) LED 
      Fluorescence Microscope Kit for the detection of Her-2/neu gene signals was 
      compared to an advanced Nikon Eclipse 80i fluorescence microscope in combination 
      with a conventional 100W mercury vapor lamp. Both microscopes were fitted with 
      the same Quicam FAST CCD digital camera to unequivocally compare the quality of 
      the captured images. C-erbB2 gene expression was analyzed in 30 different human 
      tissue samples of primary invasive breast cancer, following formalin fixation and 
      subsequent paraffin-embedding. The Her2/neu gene signals (green) were 
      identifiable in the tumor cells in all cases and images of equal quality were 
      captured under almost identical conditions by 480 nm (blue) LED module equipped 
      standard Axiostar microscope as compared to conventional fluorescence microscopy. 
      In this first attempt, these monochromatic LED elements proved in principle to be 
      suitable for the detection of Her-2/neu gene expression by FISH. Thus, our own 
      experiences emphasize the high potential of this technology to provide a serious 
      alternative to conventional fluorescence microscopy in routine pathology; 
      representing a sustainable technological progress, this low-cost technology will 
      clearly give direction also to the growing field of molecular pathology.
FAU - Lang, Dagmar S
AU  - Lang DS
AD  - Clinical and Experimental Pathology, Research Center Borstel, Parkallee 3, 
      Borstel, Germany. dlang@fz-borstel.de
FAU - Zeiser, Tobias
AU  - Zeiser T
FAU - Schultz, Holger
AU  - Schultz H
FAU - Stellmacher, Florian
AU  - Stellmacher F
FAU - Vollmer, Ekkehard
AU  - Vollmer E
FAU - Zabel, Peter
AU  - Zabel P
FAU - Goldmann, Torsten
AU  - Goldmann T
LA  - eng
PT  - Journal Article
DEP - 20081216
PL  - England
TA  - Diagn Pathol
JT  - Diagnostic pathology
JID - 101251558
PMC - PMC2615417
EDAT- 2008/12/18 09:00
MHDA- 2008/12/18 09:01
PMCR- 2008/12/16
CRDT- 2008/12/18 09:00
PHST- 2008/11/25 00:00 [received]
PHST- 2008/12/16 00:00 [accepted]
PHST- 2008/12/18 09:00 [entrez]
PHST- 2008/12/18 09:00 [pubmed]
PHST- 2008/12/18 09:01 [medline]
PHST- 2008/12/16 00:00 [pmc-release]
AID - 1746-1596-3-49 [pii]
AID - 10.1186/1746-1596-3-49 [doi]
PST - epublish
SO  - Diagn Pathol. 2008 Dec 16;3:49. doi: 10.1186/1746-1596-3-49.