PMID- 19088828
OWN - NLM
STAT- MEDLINE
DCOM- 20100601
LR  - 20171116
IS  - 1671-167X (Print)
IS  - 1671-167X (Linking)
VI  - 40
IP  - 6
DP  - 2008 Dec 18
TI  - [Establishment of a podocyte cell injury model induced by puromycin 
      aminonucleoside].
PG  - 586-9
AB  - OBJECTIVE: To establish a podocyte cell injury model induced by puromycin 
      aminonucleoside (PAN), an in vitro model for studying the role of podocytes, 
      especial the slit diaphragm molecules in proteinuria at the cellular and 
      molecular levels. METHODS: MPC5 were treated for 24 and 48 hours by 15, 45 and 75 
      mg/L PAN, respectively. The podocyte molecular behavior during podocyte injury 
      was evaluated: the apoptotic podocyte cells were revealed with FITC-Annexin V and 
      Propidium Iodide (PI) assay and the proliferative podocyte cells detected with 
      MTT assay after PAN treatment. The distribution of Nephrin and Podocin was 
      revealed with indirect-immunofluorescent staining under confocal microscope. The 
      distribution of F-actin was revealed with direct-immunofluorescent staining under 
      microscope. RESULTS: The percentage of apoptotic podocyte cells was increased in 
      a dose- and time-dependent manner after PAN treatment. In PAN-treated group, the 
      apoptosis was obviously increased at hour 48, the PAN-45 treated group was 33.48% 
      +/- 14.55% and PAN-75 treated group 38.01% +/- 12.13% vs the control group 6.38% 
      +/- 0.50% (P<0.01). CONCLUSION: We set up an in vitro podocyte injury model 
      treated with PAN for the first time. This reliable cell model is a good basis for 
      further studies on podocyte injury.
FAU - Liu, Shu Fang
AU  - Liu SF
AD  - Department of Pediatrics, Peking University First Hospital, Beijing, China.
FAU - Ding, Jie
AU  - Ding J
FAU - Fan, Qing Feng
AU  - Fan QF
FAU - Zhang, Han
AU  - Zhang H
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Beijing Da Xue Xue Bao Yi Xue Ban
JT  - Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health 
      sciences
JID - 101125284
RN  - 58-60-6 (Puromycin Aminonucleoside)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Mice
MH  - *Models, Animal
MH  - Podocytes/*pathology
MH  - *Puromycin Aminonucleoside
EDAT- 2008/12/18 09:00
MHDA- 2010/06/02 06:00
CRDT- 2008/12/18 09:00
PHST- 2008/12/18 09:00 [entrez]
PHST- 2008/12/18 09:00 [pubmed]
PHST- 2010/06/02 06:00 [medline]
PST - ppublish
SO  - Beijing Da Xue Xue Bao Yi Xue Ban. 2008 Dec 18;40(6):586-9.