PMID- 19091870
OWN - NLM
STAT- MEDLINE
DCOM- 20090216
LR  - 20211020
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 83
IP  - 5
DP  - 2009 Mar
TI  - Level of herpes simplex virus type 1 latency correlates with severity of corneal 
      scarring and exhaustion of CD8+ T cells in trigeminal ganglia of latently 
      infected mice.
PG  - 2246-54
LID - 10.1128/JVI.02234-08 [doi]
AB  - A hallmark of infection with herpes simplex virus type 1 (HSV-1) is the 
      establishment of latency in ganglia of the infected individual. During the life 
      of the latently infected individual, the virus can occasionally reactivate, 
      travel back to the eye, and cause recurrent disease. Indeed, a major cause of 
      corneal scarring (CS) is the scarring induced by HSV-1 following reactivation 
      from latency. In this study, we evaluated the relationship between the amount of 
      CS and the level of the HSV-1 latency-associated transcript (LAT) in trigeminal 
      ganglia (TG) of latently infected mice. Our results suggested that the amount of 
      CS was not related to the amount of virus replication following primary ocular 
      HSV-1 infection, since replication in the eyes was similar in mice that did not 
      develop CS, mice that developed CS in just one eye, and mice that developed CS in 
      both eyes. In contrast, mice with no CS had significantly less LAT, and thus 
      presumably less latency, in their TG than mice that had CS in both eyes. Higher 
      CS also correlated with higher levels of mRNAs for PD-1, CD4, CD8, F4/80, 
      interleukin-4, gamma interferon, granzyme A, and granzyme B in both cornea and 
      TG. These results suggest that (i) the immunopathology induced by HSV-1 infection 
      does not correlate with primary virus replication in the eye; (ii) increased CS 
      appears to correlate with increased latency in the TG, although the possible 
      cause-and-effect relationship is not known; and (iii) increased latency in mouse 
      TG correlates with higher levels of PD-1 mRNA, suggesting exhaustion of CD8+ T 
      cells.
FAU - Mott, Kevin R
AU  - Mott KR
AD  - Center for Neurobiology and Vaccine Development, Ophthalmology Research 
      Laboratories, CSMC Burns & Allen Research Institute, Los Angeles, California 
      90048, USA.
FAU - Bresee, Catherine J
AU  - Bresee CJ
FAU - Allen, Sariah J
AU  - Allen SJ
FAU - BenMohamed, Lbachir
AU  - BenMohamed L
FAU - Wechsler, Steven L
AU  - Wechsler SL
FAU - Ghiasi, Homayon
AU  - Ghiasi H
LA  - eng
GR  - R01 EY013615/EY/NEI NIH HHS/United States
GR  - R01 EY014966/EY/NEI NIH HHS/United States
GR  - EY13615/EY/NEI NIH HHS/United States
GR  - EY14966/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20081217
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antigens, Surface)
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (MicroRNAs)
RN  - 0 (Pdcd1 protein, mouse)
RN  - 0 (Programmed Cell Death 1 Receptor)
RN  - 0 (latency associated transcript, herpes simplex virus-1)
SB  - IM
MH  - Animals
MH  - Antigens, Surface/genetics/metabolism
MH  - Apoptosis Regulatory Proteins/genetics/metabolism
MH  - CD8-Positive T-Lymphocytes/metabolism/*pathology/virology
MH  - Cornea/*pathology/virology
MH  - Corneal Diseases/virology
MH  - Female
MH  - Herpes Simplex/*pathology
MH  - Herpesvirus 1, Human/genetics/*pathogenicity/physiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - MicroRNAs/genetics/metabolism
MH  - Programmed Cell Death 1 Receptor
MH  - Trigeminal Ganglion/immunology/*virology
MH  - Virus Activation
MH  - *Virus Latency
PMC - PMC2643740
EDAT- 2008/12/19 09:00
MHDA- 2009/02/17 09:00
PMCR- 2009/09/01
CRDT- 2008/12/19 09:00
PHST- 2008/12/19 09:00 [entrez]
PHST- 2008/12/19 09:00 [pubmed]
PHST- 2009/02/17 09:00 [medline]
PHST- 2009/09/01 00:00 [pmc-release]
AID - JVI.02234-08 [pii]
AID - 2234-08 [pii]
AID - 10.1128/JVI.02234-08 [doi]
PST - ppublish
SO  - J Virol. 2009 Mar;83(5):2246-54. doi: 10.1128/JVI.02234-08. Epub 2008 Dec 17.