PMID- 19091993
OWN - NLM
STAT- MEDLINE
DCOM- 20090309
LR  - 20220110
IS  - 1556-679X (Electronic)
IS  - 1556-6811 (Print)
IS  - 1556-679X (Linking)
VI  - 16
IP  - 2
DP  - 2009 Feb
TI  - Generation of immune responses against hepatitis C virus by dendritic cells 
      containing NS5 protein-coated microparticles.
PG  - 163-71
LID - 10.1128/CVI.00287-08 [doi]
AB  - Dendritic cells (DCs) internalize and process antigens as well as activate 
      cellular immune responses. The aim of this study was to determine the capacity of 
      DCs that contain antigen-coated magnetic beads to induce immunity against the 
      nonstructural hepatitis C virus (HCV) antigen 5 (NS5). Splenocytes derived from 
      Fms-like tyrosine kinase receptor 3 (Flt3) ligand-pretreated BALB/c mice were 
      incubated with magnetic beads coated with HCV NS5, lipopolysaccharide (LPS), 
      and/or anti-CD40; purified; and used for immunization. Cellular immunity was 
      measured using cytotoxic T-lymphocyte (CTL) and T-cell proliferation assays, 
      intracellular cytokine staining, and a syngeneic tumor challenge using 
      NS5-expressing SP2/0 myeloma cells in vivo. Splenocytes isolated from animals 
      vaccinated with DCs containing beads coated with NS5, LPS, and anti-CD40 secreted 
      elevated levels of interleukin-2 (IL-2) and gamma interferon in the presence of 
      NS5. The numbers of CD4(+), IL-2-producing cells were increased >5-fold in the 
      group immunized with DCs containing beads coated with NS5, LPS, and anti-CD40, 
      paralleled by an enhanced splenocyte proliferative response. Immunization 
      promoted antigen-specific CTL activity threefold compared to the level for 
      control mice and significantly reduced the growth of NS5-expressing tumor cells 
      in vivo. Thus, strategies that employ NS5-coated beads induce cellular immune 
      responses in mice, which correlate well with the natural immune responses that 
      occur in individuals who resolve HCV.
FAU - Gehring, Stephan
AU  - Gehring S
AD  - The Liver Research Center, Rhode Island Hospital and The Warren Alpert Medical 
      School of Brown University, Providence, Rhode Island 02903, USA.
FAU - Gregory, Stephen H
AU  - Gregory SH
FAU - Wintermeyer, Philip
AU  - Wintermeyer P
FAU - Aloman, Costica
AU  - Aloman C
FAU - Wands, Jack R
AU  - Wands JR
LA  - eng
GR  - CA-35711/CA/NCI NIH HHS/United States
GR  - AA-08169/AA/NIAAA NIH HHS/United States
GR  - R01 AA002666/AA/NIAAA NIH HHS/United States
GR  - R37 AA002666/AA/NIAAA NIH HHS/United States
GR  - R01 AA008169/AA/NIAAA NIH HHS/United States
GR  - AA-02666/AA/NIAAA NIH HHS/United States
GR  - R01 CA035711/CA/NCI NIH HHS/United States
GR  - R37 CA035711/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20081217
PL  - United States
TA  - Clin Vaccine Immunol
JT  - Clinical and vaccine immunology : CVI
JID - 101252125
RN  - 0 (Cytokines)
RN  - 0 (Viral Nonstructural Proteins)
RN  - EC 2.7.7.48 (NS-5 protein, hepatitis C virus)
SB  - IM
MH  - Animals
MH  - Cell Proliferation
MH  - Cytokines/biosynthesis
MH  - Cytotoxicity Tests, Immunologic
MH  - Cytotoxicity, Immunologic
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Hepacivirus/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - *Microspheres
MH  - T-Lymphocytes/immunology
MH  - Viral Nonstructural Proteins/*immunology
PMC - PMC2643538
EDAT- 2008/12/19 09:00
MHDA- 2009/03/10 09:00
PMCR- 2009/08/01
CRDT- 2008/12/19 09:00
PHST- 2008/12/19 09:00 [entrez]
PHST- 2008/12/19 09:00 [pubmed]
PHST- 2009/03/10 09:00 [medline]
PHST- 2009/08/01 00:00 [pmc-release]
AID - CVI.00287-08 [pii]
AID - 0287-08 [pii]
AID - 10.1128/CVI.00287-08 [doi]
PST - ppublish
SO  - Clin Vaccine Immunol. 2009 Feb;16(2):163-71. doi: 10.1128/CVI.00287-08. Epub 2008 
      Dec 17.