PMID- 19093256
OWN - NLM
STAT- MEDLINE
DCOM- 20090127
LR  - 20081218
IS  - 1532-4192 (Electronic)
IS  - 0735-7907 (Linking)
VI  - 26
IP  - 10
DP  - 2008 Dec
TI  - Inhibition of laryngeal cancer cell invasion and growth with lentiviral-vector 
      delivered short hairpin RNA targeting human MMP-9 gene.
PG  - 984-9
LID - 10.1080/07357900802072897 [doi]
AB  - The purpose of this study was to explore the inhibiting role of MMP-9 gene 
      silence in the invasive ability and growth of laryngeal squamous cell carcinoma 
      (LSCC) by lentivirus mediated RNA interference. MMP-9-RNAi-lentivirus and the 
      control lentivirus (GFP-lentivirus) were transfected into Hep-2 cells. Gelatin 
      zymography showed the proteins expression of MMP-9 were knockdown in the MMP-9 
      siRNA transfected Hep-2 cells. The invasive activity and viability of MMP-9 siRNA 
      treated Hep-2 cells were decreased than the control cells measured with modified 
      Boyden chamber assay and MTT assay. In animal experiment, 20 nude mice bearing 
      Hep-2 cell tumor were randomly separated into the experimental and the control 
      groups. The former were intratumorally injected with MMP-9-RNAi-lentivirus, and 
      the later were injected with equivalent dose of GFP-lentivirus. Results showed 
      the average weight and volume of tumor in MMP-9-RNAi-lentivirus treated group 
      were significantly lower than those in the control group (P < .01). The protein 
      expressions of MMP-9 were downregulated in tumors of MMP-9-RNAi-lentivirus 
      treatment. The PCNA index was obviously lower in the tumors of treated group than 
      that in the control group (P < .01). These results suggest that MMP-9 gene 
      silence by lentivirus mediated RNA interference can inhibit invasion and growth 
      of LSCC.
FAU - Sun, Yanan
AU  - Sun Y
AD  - The Department of Otorhinolaryngology, Head and Neck Surgery, The Second 
      Affiliated Hospital, Harbin Medical University, Harbin, PR China.
FAU - Liu, Ming
AU  - Liu M
FAU - Yang, Baofeng
AU  - Yang B
FAU - Lu, Jianguang
AU  - Lu J
FAU - Li, Baoxin
AU  - Li B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cancer Invest
JT  - Cancer investigation
JID - 8307154
RN  - 0 (RNA, Catalytic)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Animals
MH  - Carcinoma, Squamous Cell/enzymology/genetics/*pathology
MH  - Cell Division
MH  - Cell Line, Tumor
MH  - Gene Expression Regulation, Enzymologic
MH  - Gene Expression Regulation, Neoplastic
MH  - Gene Silencing
MH  - Genetic Vectors
MH  - Humans
MH  - Laryngeal Neoplasms/*enzymology/genetics/*pathology
MH  - Lentivirus/*genetics
MH  - Matrix Metalloproteinase 9/*genetics
MH  - Mice
MH  - Mice, Nude
MH  - Neoplasm Invasiveness
MH  - *RNA Interference
MH  - RNA, Catalytic/*genetics
MH  - Transfection
EDAT- 2008/12/19 09:00
MHDA- 2009/01/28 09:00
CRDT- 2008/12/19 09:00
PHST- 2008/12/19 09:00 [entrez]
PHST- 2008/12/19 09:00 [pubmed]
PHST- 2009/01/28 09:00 [medline]
AID - 906934709 [pii]
AID - 10.1080/07357900802072897 [doi]
PST - ppublish
SO  - Cancer Invest. 2008 Dec;26(10):984-9. doi: 10.1080/07357900802072897.