PMID- 19095579
OWN - NLM
STAT- MEDLINE
DCOM- 20090317
LR  - 20081219
IS  - 1896-1126 (Print)
IS  - 1896-1126 (Linking)
VI  - 53
IP  - 2
DP  - 2008
TI  - Quantification of the mRNA encoding Tumor Necrosis Factor alpha (TNFalpha) and 
      its receptors in human nasal polyps.
PG  - 263-9
LID - 10.2478/v10039-008-0051-7 [doi]
AB  - PURPOSE: The object of the study was to assess the expression of the genes 
      encoding TNFalpha and its receptors (TNF-R1 and TNF-R2) in patients with nasal 
      polyps (NP). MATERIAL AND METHODS: The number of the mRNA copies was assessed by 
      QRT-PCR in RNA extracts from 16 eosinophilic (ENP) and 5 neutrophilic nasal 
      polyps (NNP), and 9 normal mucosa (NM) samples. The expression of corresponding 
      proteins was demonstrated using immunohistochemistry. RESULTS: The mean level of 
      mRNA copies for TNFalpha in ENP (82229c/microg) was not significantly higher when 
      compared with controls (74869c/microg). NNP demonstrated significantly lower mean 
      TNFalpha gene expression (7021c/microg) than the controls (p<0.05). A 
      statistically higher mRNA TNFalpha copy number in ENP than in NNP was also 
      revealed (p<0.01). A noticeably lower mRNA expression of TNF-R1 in ENP and NNP 
      was seen as compared to the control group (10198c/microg vs. 30749c/microg, 
      p<0.05 and 3440c/microg vs. 30749c/microg; p<0.05 respectively). In ENP the mean 
      TNF-R2 mRNA copy number was markedly higher than in NNP (185c/microg vs. 
      7.6c/microg, p<0.05). TNF-R2 mRNA level did not differ significantly between ENP 
      and the control group (185c/microg vs. 469c/microg). TNF-R1 expression was 
      significantly higher than TNF-R2 at the mRNA (p<0.01) and protein (p<0.05) level 
      both in ENP and NNP. No significant correlations in proteins expression were 
      detected between ENP and NNP. CONCLUSIONS: TNF-R1 has been identified to be a 
      prevalent form of the TNFalpha receptor in nasal polyps which may reflect the 
      apparent dominance of this form in TNFalpha signalling. The findings raise the 
      possibility that the eosinophils from NP may influence biological responses 
      through TNFalpha-dependent mechanisms. The differences between ENP and NNP 
      relating to TNFalpha and the expression of its receptors may reflect the distinct 
      character of those diseases.
FAU - Rostkowska-Nadolska, B
AU  - Rostkowska-Nadolska B
AD  - Department of Otolaryngology, Wroclaw Medical University, Wroclaw, Poland.
FAU - Kapral, M
AU  - Kapral M
FAU - Mazurek, U
AU  - Mazurek U
FAU - Fraczek, M
AU  - Fraczek M
FAU - Ziolkowski, P
AU  - Ziolkowski P
FAU - Gamian, E
AU  - Gamian E
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Adv Med Sci
JT  - Advances in medical sciences
JID - 101276222
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Tumor Necrosis Factor, Type I)
RN  - 0 (Receptors, Tumor Necrosis Factor, Type II)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Chronic Disease
MH  - Eosinophils/metabolism/pathology
MH  - Female
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Male
MH  - Middle Aged
MH  - Nasal Polyps/*genetics
MH  - Neutrophils/metabolism/pathology
MH  - RNA, Messenger/*genetics/metabolism
MH  - Receptors, Tumor Necrosis Factor, Type I/*genetics/metabolism
MH  - Receptors, Tumor Necrosis Factor, Type II/*genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Rhinitis/diagnosis/genetics/metabolism
MH  - Sinusitis/diagnosis/genetics/metabolism
MH  - Tumor Necrosis Factor-alpha/*genetics/metabolism
EDAT- 2008/12/20 09:00
MHDA- 2009/03/18 09:00
CRDT- 2008/12/20 09:00
PHST- 2008/12/20 09:00 [entrez]
PHST- 2008/12/20 09:00 [pubmed]
PHST- 2009/03/18 09:00 [medline]
AID - 0K2441K15836316G [pii]
AID - 10.2478/v10039-008-0051-7 [doi]
PST - ppublish
SO  - Adv Med Sci. 2008;53(2):263-9. doi: 10.2478/v10039-008-0051-7.