PMID- 19096206
OWN - NLM
STAT- MEDLINE
DCOM- 20090112
LR  - 20211020
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Print)
IS  - 1424-8581 (Linking)
VI  - 122
IP  - 2
DP  - 2008
TI  - A genome assembly-integrated dog 1 Mb BAC microarray: a cytogenetic resource for 
      canine cancer studies and comparative genomic analysis.
PG  - 110-21
LID - 10.1159/000163088 [doi]
AB  - Molecular cytogenetic studies have been instrumental in defining the nature of 
      numerical and structural chromosome changes in human cancers, but their 
      significance remains to be fully understood. The emergence of high quality genome 
      assemblies for several model organisms provides exciting opportunities to develop 
      novel genome-integrated molecular cytogenetic resources that now permit a 
      comparative approach to evaluating the relevance of tumor-associated chromosome 
      aberrations, both within and between species. We have used the dog genome 
      sequence assembly to identify a framework panel of 2,097 bacterial artificial 
      chromosome (BAC) clones, selected at intervals of approximately one megabase. 
      Each clone has been evaluated by multicolor fluorescence in situ hybridization 
      (FISH) to confirm its unique cytogenetic location in concordance with its 
      reported position in the genome assembly, providing new information on the 
      organization of the dog genome. This panel of BAC clones also represents a 
      powerful cytogenetic resource with numerous potential applications. We have used 
      the clone set to develop a genome-wide microarray for comparative genomic 
      hybridization (aCGH) analysis, and demonstrate its application in detection of 
      tumor-associated DNA copy number aberrations (CNAs) including single copy 
      deletions and amplifications, regional aneuploidy and whole chromosome 
      aneuploidy. We also show how individual clones selected from the BAC panel can be 
      used as FISH probes in direct evaluation of tumor karyotypes, to verify and 
      explore CNAs detected using aCGH analysis. This cytogenetically validated, genome 
      integrated BAC clone panel has enormous potential for aiding gene discovery 
      through a comparative approach to molecular oncology.
CI  - Copyright 2008 S. Karger AG, Basel.
FAU - Thomas, R
AU  - Thomas R
AD  - Department of Molecular Biomedical Sciences, College of Veterinary Medicine, 
      Raleigh, NC 27606, USA.
FAU - Duke, S E
AU  - Duke SE
FAU - Karlsson, E K
AU  - Karlsson EK
FAU - Evans, A
AU  - Evans A
FAU - Ellis, P
AU  - Ellis P
FAU - Lindblad-Toh, K
AU  - Lindblad-Toh K
FAU - Langford, C F
AU  - Langford CF
FAU - Breen, M
AU  - Breen M
LA  - eng
GR  - R21 NS051190/NS/NINDS NIH HHS/United States
GR  - R21 NS051190-02/NS/NINDS NIH HHS/United States
GR  - WT_/Wellcome Trust/United Kingdom
GR  - R21 NS051190-01/NS/NINDS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20081218
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
SB  - IM
MH  - Animals
MH  - Chromosomes/genetics
MH  - Comparative Genomic Hybridization
MH  - Cytogenetics
MH  - Databases, Nucleic Acid
MH  - Dogs
MH  - Genome/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Neoplasms/*genetics
MH  - Oligonucleotide Array Sequence Analysis
PMC - PMC2874680
EDAT- 2008/12/20 09:00
MHDA- 2009/01/13 09:00
PMCR- 2008/12/01
CRDT- 2008/12/20 09:00
PHST- 2008/07/17 00:00 [accepted]
PHST- 2008/12/20 09:00 [entrez]
PHST- 2008/12/20 09:00 [pubmed]
PHST- 2009/01/13 09:00 [medline]
PHST- 2008/12/01 00:00 [pmc-release]
AID - 000163088 [pii]
AID - cgr0122-0110 [pii]
AID - 10.1159/000163088 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2008;122(2):110-21. doi: 10.1159/000163088. Epub 2008 Dec 
      18.