PMID- 19096460
OWN - NLM
STAT- MEDLINE
DCOM- 20090402
LR  - 20081219
IS  - 0008-4166 (Print)
IS  - 0008-4166 (Linking)
VI  - 54
IP  - 12
DP  - 2008 Dec
TI  - Spatial distribution of bacterial communities in sediment of a eutrophic lake 
      revealed by denaturing gradient gel electrophoresis and multivariate analysis.
PG  - 1053-63
LID - 10.1139/W08-098 [doi]
AB  - Bacterial community structure and the effects of several environmental factors on 
      the microbial community distribution were investigated in the sediment of the 
      eutrophic Lake Xuanwu. Profiles of bacterial communities were generated using 
      denaturing gradient gel electrophoresis (DGGE), and the results were interpreted 
      with multivariate statistical analysis. Five major variables in sediment were 
      examined in a principal component analysis, which indicates notable differences 
      of physicochemical parameters among different sites of the lake. To assess 
      changes in the genetic diversity of bacterial communities of different sampling 
      sites, DGGE band patterns were analyzed by multidimensional scaling analysis, 
      which indicated that sampling sites having similar environmental characteristics 
      also have the similar microbial communities. Canonical correspondence analysis 
      demonstrated that pH and redox potential had significant effects on the bacterial 
      community composition in the sediments. Analysis of DNA sequences revealed that 
      the dominant bacterial groups in Lake Xuanwu belonged to Proteobacteria, 
      Actinobacteria, Verrucomicrobia, and Nitrospirae, which are commonly isolated 
      from freshwater ecosystems.
FAU - Zeng, Jin
AU  - Zeng J
AD  - State Key Laboratory of Pollution Control and Resource Reuse, School of the 
      Environment, Nanjing University, Nanjing 210093, China.
FAU - Yang, Liu-Yan
AU  - Yang LY
FAU - Liang, Yi
AU  - Liang Y
FAU - Li, Jia-Yun
AU  - Li JY
FAU - Xiao, Lin
AU  - Xiao L
FAU - Jiang, Li-Juan
AU  - Jiang LJ
FAU - Zhao, Da-Yong
AU  - Zhao DY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Can J Microbiol
JT  - Canadian journal of microbiology
JID - 0372707
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Actinobacteria/classification/genetics/*isolation & purification
MH  - Alphaproteobacteria/classification/genetics/*isolation & purification
MH  - Betaproteobacteria/classification/genetics/*isolation & purification
MH  - China
MH  - Chlamydiales/classification/genetics/*isolation & purification
MH  - DNA, Bacterial/classification/genetics/*isolation & purification
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Eutrophication
MH  - *Fresh Water
MH  - Genetic Variation
MH  - Geologic Sediments/*analysis/*microbiology
MH  - Multivariate Analysis
MH  - Nucleic Acid Denaturation
MH  - Phylogeny
MH  - Sequence Analysis, DNA
MH  - *Water Microbiology
EDAT- 2008/12/20 09:00
MHDA- 2009/04/03 09:00
CRDT- 2008/12/20 09:00
PHST- 2008/12/20 09:00 [entrez]
PHST- 2008/12/20 09:00 [pubmed]
PHST- 2009/04/03 09:00 [medline]
AID - w08-098 [pii]
AID - 10.1139/W08-098 [doi]
PST - ppublish
SO  - Can J Microbiol. 2008 Dec;54(12):1053-63. doi: 10.1139/W08-098.