PMID- 19097411
OWN - NLM
STAT- MEDLINE
DCOM- 20090122
LR  - 20100331
IS  - 0862-495X (Print)
IS  - 0862-495X (Linking)
VI  - 21
IP  - 1
DP  - 2008
TI  - [Comparison of dendritic cells antigens in healthy volunteers and monoclonal 
      gammopathy of undetermined significance and/or multiple myeloma patients].
PG  - 20-5
AB  - BACKGROUND: Dendritic cells (DCs) are highly specialized antigen-presenting 
      cells, which can be used for immunotherapy trials. Functionally normal DCs play a 
      critical role in the activation and potentiation of antitumor antigen-specific 
      responses. DESIGN AND SUBJECTS: Maturation of DCs from 10 healthy donors, 14 
      monoclonal gammopathy of undetermined significance patients and 14 multiple 
      myeloma patients was tested in an in vitro study. METHODS AND RESULTS: DCs were 
      generated from adherent mononuclear precursors of peripheral blood and cultured 
      in presence of IL-4 and GM-CSF with human CD40Ligand stimulation. Serum-free or 
      autologous serum conditions were used and expression of significant surface 
      antigens, chemokines receptors and production of IL-12p70, were compared. We 
      found no difference between groups under serum-free conditions with or without 
      CD40L stimulation. Under autologous conditions we found negative effect on 
      patients DCs manifested by reduction of some markers. The production of IL-12p70 
      was low and no difference in serum IL-6 levels between individual groups was 
      found. CONCLUSION: Under serum free conditions there was no difference between 
      healthy volunteers, MGUS and patients, but CD40L stimulation did not lead to the 
      full maturation ofDCs. Autologous patient serum had negative influence on DCs, 
      with no definite dependance on the IL-6 level.
FAU - Kovarova, L
AU  - Kovarova L
AD  - Oddeleni Klinicke Hematologie - Laborator Experimentalni Hematologie a Bunecne 
      Imunoterapie (LEHABI), FN Brno, PMDV, Jihlavska 20, 625 00 Brno. 
      lkovarova@fnbrno.cz
FAU - Michalek, J
AU  - Michalek J
FAU - Kyr, M
AU  - Kyr M
FAU - Penka, M
AU  - Penka M
FAU - Hajek, R
AU  - Hajek R
LA  - cze
PT  - Comparative Study
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
TT  - Srovnani vybranych markeru dendritickych bunek u zdravych darcu a pacientu s 
      monoklonalni gamapatii nejasneho vyznamu ci mnohocetnym myelomem.
PL  - Czech Republic
TA  - Klin Onkol
JT  - Klinicka onkologie : casopis Ceske a Slovenske onkologicke spolecnosti
JID - 9425213
RN  - 0 (Antigens, Surface)
RN  - 0 (Interleukin-6)
RN  - 0 (Receptors, CCR)
RN  - 147205-72-9 (CD40 Ligand)
SB  - IM
MH  - Antigens, Surface/metabolism
MH  - CD40 Ligand/pharmacology
MH  - Dendritic Cells/*immunology
MH  - Humans
MH  - Interleukin-6/metabolism
MH  - Middle Aged
MH  - Multiple Myeloma/*immunology
MH  - Paraproteinemias/*immunology
MH  - Receptors, CCR/metabolism
EDAT- 2008/12/23 09:00
MHDA- 2009/01/23 09:00
CRDT- 2008/12/23 09:00
PHST- 2008/12/23 09:00 [entrez]
PHST- 2008/12/23 09:00 [pubmed]
PHST- 2009/01/23 09:00 [medline]
PST - ppublish
SO  - Klin Onkol. 2008;21(1):20-5.