PMID- 19098046
OWN - NLM
STAT- MEDLINE
DCOM- 20090212
LR  - 20191210
IS  - 1468-2079 (Electronic)
IS  - 0007-1161 (Linking)
VI  - 93
IP  - 1
DP  - 2009 Jan
TI  - Interference microscopy delineates cellular proliferations on flat mounted 
      internal limiting membrane specimens.
PG  - 120-2
LID - 10.1136/bjo.2008.146514 [doi]
AB  - AIM: To demonstrate that interference microscopy of flat mounted internal 
      limiting membrane specimens clearly delineates cellular proliferations at the 
      vitreomacular interface. METHODS: ILM specimens harvested during vitrectomy were 
      fixed in glutaraldehyde 0.05% and paraformaldehyde 2% for 24 h (pH 7.4). In 
      addition to interference microscopy, immunocytochemistry using antibodies against 
      glial fibrillar acidic protein (GFAP) and neurofilament (NF) was performed. After 
      washing in phosphate-buffered saline 0.1 M, the specimens were flat-mounted on 
      glass slides without sectioning, embedding or any other technique of conventional 
      light microscopy. A cover slide and 4',6-diamidino-2-phenylindole (DAPI) medium 
      were added to stain the cell nuclei. RESULTS: Interference microscopy clearly 
      delineates cellular proliferations at the ILM. DAPI stained the cell nuclei. 
      Areas of cellular proliferation can be easily distinguished from ILM areas 
      without cells. Immunocytochemistry can be performed without changing the 
      protocols used in conventional microscopy. CONCLUSION: Interference microscopy of 
      flat mounted ILM specimens gives new insights into the distribution of cellular 
      proliferations at the vitreomacular interface and allows for determination of the 
      cell density at the ILM. Given that the entire ILM peeled is seen en face, the 
      techniques described offer a more reliable method to investigate the 
      vitreoretinal interface in terms of cellular distribution compared with 
      conventional microscopy.
FAU - Gandorfer, A
AU  - Gandorfer A
AD  - Vitreoretinal and Pathology Unit, University Eye Hospital Munich, Mathildenstr. 
      8, 80336 Munich, Germany. arnd.gandorfer@med.uni-muenchen.de
FAU - Scheler, R
AU  - Scheler R
FAU - Schumann, R
AU  - Schumann R
FAU - Haritoglou, C
AU  - Haritoglou C
FAU - Kampik, A
AU  - Kampik A
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - England
TA  - Br J Ophthalmol
JT  - The British journal of ophthalmology
JID - 0421041
SB  - IM
MH  - Cell Count
MH  - *Cell Proliferation
MH  - Cells, Cultured
MH  - Epiretinal Membrane/*pathology
MH  - Humans
MH  - Immunohistochemistry
MH  - *Microscopy, Interference
MH  - Retinal Ganglion Cells/*cytology
MH  - Vitrectomy/methods
EDAT- 2008/12/23 09:00
MHDA- 2009/02/13 09:00
CRDT- 2008/12/23 09:00
PHST- 2008/12/23 09:00 [entrez]
PHST- 2008/12/23 09:00 [pubmed]
PHST- 2009/02/13 09:00 [medline]
AID - 93/1/120 [pii]
AID - 10.1136/bjo.2008.146514 [doi]
PST - ppublish
SO  - Br J Ophthalmol. 2009 Jan;93(1):120-2. doi: 10.1136/bjo.2008.146514.