PMID- 19098700
OWN - NLM
STAT- MEDLINE
DCOM- 20110325
LR  - 20091112
IS  - 1476-5454 (Electronic)
IS  - 0950-222X (Linking)
VI  - 23
IP  - 11
DP  - 2009 Nov
TI  - Characterization of dendritic cell phenotype in allergic conjunctiva: increased 
      expression of Fc(epsilon)RI, the high-affinity receptor for immunoglobulin E.
PG  - 2099-104
LID - 10.1038/eye.2008.372 [doi]
AB  - PURPOSE: Dendritic cells (DCs) express the high-affinity receptor for IgE 
      (Fc(epsilon)RI) on their surface, which may enhance their ability to capture and 
      internalize antigens for presentation to T-lymphocytes. The aim of this study was 
      to determine if expression of Fc(epsilon)RI(+) DCs is increased in the 
      conjunctivae of vernal keratoconjunctivitis (VKC) patients compared with those of 
      normal controls. METHODS: Conjunctival biopsies were obtained from non-atopic and 
      VKC patients. Double immunohistochemical staining was carried out using 
      antibodies against Fc(epsilon)RI and the CD1a antigen, a DC marker. The 
      double-positive cells were counted in five representative fields of view for each 
      conjunctival sample. RESULTS: Fc(epsilon)RI(+) CD1a(+) cells were present in 
      significantly higher numbers in VKC conjunctivae compared with normal controls 
      (mean cell count of 21.3 in VKC vs5.0 in controls, P<0.005). In normal patients 
      the Fc(epsilon)RI-expressing DCs tended to be confined to the epithelial layer or 
      the superficial substantia propria, but in the VKC samples these Fc(epsilon)RI(+) 
      cells were mainly concentrated in the deeper substantia propria. CONCLUSIONS: 
      Fc(epsilon)RI(+) DC numbers are elevated in the conjunctivae of VKC patients, a 
      finding consistent with the results of other studies focusing on atopic 
      conditions. Elevated expression of Fc(epsilon)RI on DCs would facilitate antigen 
      presentation and enhance T-cell priming, thereby contributing to ocular symptoms.
FAU - Manzouri, B
AU  - Manzouri B
AD  - UCL, Institute of Ophthalmology, London, UK. b.manzouri@ucl.ac.uk
FAU - Ohbayashi, M
AU  - Ohbayashi M
FAU - Leonardi, A
AU  - Leonardi A
FAU - Larkin, D F P
AU  - Larkin DF
FAU - Ono, S J
AU  - Ono SJ
LA  - eng
GR  - Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081219
PL  - England
TA  - Eye (Lond)
JT  - Eye (London, England)
JID - 8703986
RN  - 0 (Receptors, IgE)
SB  - IM
MH  - Conjunctiva/metabolism
MH  - Conjunctivitis, Allergic/*metabolism
MH  - Dendritic Cells/cytology/*metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Phenotype
MH  - Receptors, IgE/*metabolism
EDAT- 2008/12/23 09:00
MHDA- 2011/03/26 06:00
CRDT- 2008/12/23 09:00
PHST- 2008/12/23 09:00 [entrez]
PHST- 2008/12/23 09:00 [pubmed]
PHST- 2011/03/26 06:00 [medline]
AID - eye2008372 [pii]
AID - 10.1038/eye.2008.372 [doi]
PST - ppublish
SO  - Eye (Lond). 2009 Nov;23(11):2099-104. doi: 10.1038/eye.2008.372. Epub 2008 Dec 
      19.