PMID- 19098936
OWN - NLM
STAT- MEDLINE
DCOM- 20090421
LR  - 20211020
IS  - 1745-7254 (Electronic)
IS  - 1671-4083 (Print)
IS  - 1671-4083 (Linking)
VI  - 30
IP  - 1
DP  - 2009 Jan
TI  - PPAR-gamma agonists inhibit TGF-beta1-induced chemokine expression in human 
      tubular epithelial cells.
PG  - 107-12
LID - 10.1038/aps.2008.15 [doi]
AB  - AIM: Peroxisome proliferator-activated receptor-gamma (PPAR-gamma) has a wide 
      range of biological functions, including anti-inflammation. In this study, we 
      investigated the inhibitory effects of PPAR-gamma on transforming growth factor 
      beta1 (TGF-beta1)-induced interleukin-8 (IL-8) and monocyte chemoattractant 
      protein-1 (MCP-1) expression in renal tubular epithelial cells (HK-2). METHODS: 
      HK-2 cells were pretreated with 15d-PGJ2 or troglitazone (TGL) and then treated 
      with TGF-beta1. Expression of MCP-1 and IL-8 was measured using real-time PCR and 
      ELISA. RESULTS: Treatment with 5 ng/mL TGF-beta1 for 24 h increased both MCP-1 
      and IL-8 mRNA and protein levels in HK-2 cells. Both 15d-PGJ2 at 2.5 and 5 
      micromol/L and TGL at 2.5 micromol/L exhibited inhibitory effects on 
      TGF-beta1-induced MCP-1 expression. Additionally, 15d-PGJ2 at 2.5 and 5 
      micromol/L and TGL at 2.5 micromol/L inhibited TGF-beta1-induced expression of 
      IL-8. CONCLUSION: PPAR-gamma agonists (15d-PGJ2 and TGL) could inhibit the 
      TGF-beta1-induced expression of chemokines in HK-2 cells. Our results suggest 
      that PPAR-gamma agonists have the potential to be used as a treatment regimen to 
      reduce inflammation in renal tubulointerstitial disease.
FAU - Wang, Wei-ming
AU  - Wang WM
AD  - Department of Nephrology, Ruijin Hospital, Shanghai Jiao Tong University, School 
      of Medicine, Shanghai 200025, China.
FAU - Zhang, Hui-di
AU  - Zhang HD
FAU - Jin, Yuan-meng
AU  - Jin YM
FAU - Zhu, Bing-bing
AU  - Zhu BB
FAU - Chen, Nan
AU  - Chen N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081222
PL  - United States
TA  - Acta Pharmacol Sin
JT  - Acta pharmacologica Sinica
JID - 100956087
RN  - 0 (15-deoxyprostaglandin J2)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chromans)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Interleukin-8)
RN  - 0 (PPAR gamma)
RN  - 0 (Thiazolidinediones)
RN  - 0 (Transforming Growth Factor beta1)
RN  - I66ZZ0ZN0E (Troglitazone)
RN  - RXY07S6CZ2 (Prostaglandin D2)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chemokine CCL2/genetics/*immunology
MH  - Chromans/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Epithelial Cells/cytology/*drug effects/*immunology
MH  - Humans
MH  - Hypoglycemic Agents/pharmacology
MH  - Interleukin-8/genetics/*immunology
MH  - Kidney Tubules, Proximal/cytology/*immunology
MH  - PPAR gamma/*agonists/immunology
MH  - Prostaglandin D2/analogs & derivatives/metabolism
MH  - Thiazolidinediones/pharmacology
MH  - Transforming Growth Factor beta1/immunology/*pharmacology
MH  - Troglitazone
PMC - PMC4006532
EDAT- 2008/12/23 09:00
MHDA- 2009/04/22 09:00
PMCR- 2009/01/01
CRDT- 2008/12/23 09:00
PHST- 2008/12/23 09:00 [entrez]
PHST- 2008/12/23 09:00 [pubmed]
PHST- 2009/04/22 09:00 [medline]
PHST- 2009/01/01 00:00 [pmc-release]
AID - aps200815 [pii]
AID - 10.1038/aps.2008.15 [doi]
PST - ppublish
SO  - Acta Pharmacol Sin. 2009 Jan;30(1):107-12. doi: 10.1038/aps.2008.15. Epub 2008 
      Dec 22.