PMID- 19099236
OWN - NLM
STAT- MEDLINE
DCOM- 20090203
LR  - 20171116
IS  - 0253-6269 (Print)
IS  - 0253-6269 (Linking)
VI  - 31
IP  - 12
DP  - 2008 Dec
TI  - Simultaneous determination of methamphetamine, 
      3,4-methylenedioxy-N-methylamphetamine, 3,4-methylenedioxy-N-ethylamphetamine, 
      N,N-dimethylamphetamine, and their metabolites in urine by liquid 
      chromatography-electrospray ionization-tandem mass spectrometry.
PG  - 1644-51
LID - 10.1007/s12272-001-2163-5 [doi]
AB  - A liquid chromatography-electrospray ionization-tandem mass spectrometric 
      (LC-ESI-MS/MS) method was developed and validated for the simultaneous detection 
      and quantification of seven amphetamine derivatives (amphetamine (AP), 
      methamphetamine (MA), 3,4-methylenedioxy-N-amphetamine (MDA), 
      3,4-methylenedioxy-N-methamphetamine (MDMA), 
      3,4-methylenedioxy-N-ethylamphetamine (MDEA), N,N-dimethylamphetamine (DMA), and 
      N,N-dimethylamphetamine-N-oxide (DMANO)) in human urine. Seven deuterium-labeled 
      compounds were prepared for use as internal standards to quantify the analytes. 
      One milliliter of urine was combined with 1 mL of 0.2 M sodium carbonate buffer 
      solution (pH 9.0) before solid phase extraction (SPE). An Oasis HLB SPE column 
      followed by chromatographic separation on a Capcell Pak C18 MG-II column (150 x 
      2.0 mm I.D., 5 microm) and electrospray mass spectrometry with multiple reaction 
      monitoring were used for selective and sensitive detection. The use of ammonium 
      formate (5 mM, pH adjusted to 4.0 with formic acid, Solvent A) and acetonitrile 
      (Solvent B) as the mobile phase at a flow rate of 230 microL/min was found to be 
      the most effective for the separation. The linear ranges were 5.0-1000 ng/mL for 
      AP, MDA, MDMA, MDEA, DMA, and DMANO and 10.0-1000 ng/mL for MA, with good 
      correlation coefficients (r2 > 0.996). The intra-day, inter-day, and interperson 
      precisions were within 14.6%, 12.1% and 15.5%, respectively. The intra-day, 
      inter-day, and interperson accuracies were between -11.6 and 9.0%, -7.9 and 2.3%, 
      and -13.2 and 4.3%, respectively. The limits of detection (LODs) for each 
      analytical compound were lower than 1.95 ng/mL. The recovery ranged from 72.3 to 
      103.3%. The applicability of the developed method was examined by analyzing 
      several urine samples from confirmed drug abusers.
FAU - Kim, Jin Young
AU  - Kim JY
AD  - Drug Analysis Laboratory, Forensic Science Division, Supreme Prosecutors' Office, 
      Seoul, 137-730, Korea. paxus@spo.go.kr
FAU - Cheong, Jae Chul
AU  - Cheong JC
FAU - Ko, Beom Jun
AU  - Ko BJ
FAU - Lee, Sang Kyu
AU  - Lee SK
FAU - Yoo, Hye Hyun
AU  - Yoo HH
FAU - Jin, Changbae
AU  - Jin C
FAU - In, Moon Kyo
AU  - In MK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081220
PL  - Korea (South)
TA  - Arch Pharm Res
JT  - Archives of pharmacal research
JID - 8000036
RN  - 0 (Amphetamines)
RN  - 0 (Indicators and Reagents)
RN  - 44RAL3456C (Methamphetamine)
RN  - 4764-17-4 (3,4-Methylenedioxyamphetamine)
RN  - IYE3F3MHCU (dimethylamphetamine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
RN  - ML1I4KK67B (3,4-methylenedioxyethamphetamine)
SB  - IM
MH  - 3,4-Methylenedioxyamphetamine/analogs & derivatives/urine
MH  - Amphetamines/*urine
MH  - Chromatography, High Pressure Liquid
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Indicators and Reagents
MH  - Methamphetamine/analogs & derivatives/urine
MH  - N-Methyl-3,4-methylenedioxyamphetamine/urine
MH  - Reproducibility of Results
MH  - Spectrometry, Mass, Electrospray Ionization
EDAT- 2008/12/23 09:00
MHDA- 2009/02/04 09:00
CRDT- 2008/12/23 09:00
PHST- 2008/09/19 00:00 [received]
PHST- 2008/11/14 00:00 [accepted]
PHST- 2008/11/07 00:00 [revised]
PHST- 2008/12/23 09:00 [entrez]
PHST- 2008/12/23 09:00 [pubmed]
PHST- 2009/02/04 09:00 [medline]
AID - 10.1007/s12272-001-2163-5 [doi]
PST - ppublish
SO  - Arch Pharm Res. 2008 Dec;31(12):1644-51. doi: 10.1007/s12272-001-2163-5. Epub 
      2008 Dec 20.