PMID- 1909938
OWN - NLM
STAT- MEDLINE
DCOM- 19911023
LR  - 20190510
IS  - 0143-3334 (Print)
IS  - 0143-3334 (Linking)
VI  - 12
IP  - 9
DP  - 1991 Sep
TI  - Constitutively elevated levels of ornithine and polyamines in mouse epidermal 
      papillomas.
PG  - 1619-25
AB  - Epidermal papillomas were induced in CD-1 mice by a single topical application of 
      7,12-dimethylbenzanthracene (DMBA) followed by twice weekly applications of 
      12-O-tetradecanoyl-phorbol-13-acetate (TPA) in acetone. Control groups consisted 
      of mice treated singly or chronically with acetone or TPA. TPA induced a rapid, 
      yet transient 500- to 1000-fold increase in ornithine decarboxylase (ODC) 
      activity which resulted in a 2- to 8.4-fold elevation of putrescine in both 
      singly or chronically TPA-treated mouse epidermis 4-6 h after its application. 
      After 24 h, levels of spermidine, but not spermine, were also elevated. The ODC 
      and arginase activities in the 11 individual papillomas studied averaged 400- and 
      26-fold higher respectively than basal levels in epidermis. The activity of ODC 
      in most papillomas, unlike ODC in epidermis, could be stimulated by guanosine 
      5'-triphosphate (GTP). Putrescine and spermidine levels in papillomas, especially 
      those exhibiting highly GTP-stimulated ODC, were substantially higher compared to 
      either normal or TPA-treated epidermis. Although epidermis contains a relatively 
      high ornithine content, its level is even further elevated in papillomas, in some 
      cases as much as 70-fold. The consequences of the constitutively elevated 
      polyamine levels in papillomas caused by the loss of control over the normally 
      tightly regulated polyamine biosynthetic pathway are not known, but could be 
      important in regulating the balance between proliferation and differentiation in 
      this self-renewing epithelial tissue.
FAU - Koza, R A
AU  - Koza RA
AD  - Lankenau Medical Research Center, Philadelphia, PA 19151.
FAU - Megosh, L C
AU  - Megosh LC
FAU - Palmieri, M
AU  - Palmieri M
FAU - O'Brien, T G
AU  - O'Brien TG
LA  - eng
GR  - ES-01664/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Carcinogenesis
JT  - Carcinogenesis
JID - 8008055
RN  - 0 (Polyamines)
RN  - 57-97-6 (9,10-Dimethyl-1,2-benzanthracene)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - E524N2IXA3 (Ornithine)
RN  - EC 4.1.1.17 (Ornithine Decarboxylase)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - 9,10-Dimethyl-1,2-benzanthracene/toxicity
MH  - Animals
MH  - Female
MH  - Guanosine Triphosphate/metabolism
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Ornithine/*analysis
MH  - Ornithine Decarboxylase/metabolism
MH  - Papilloma/chemically induced/*chemistry
MH  - Polyamines/*analysis
MH  - Skin Neoplasms/chemically induced/*chemistry
MH  - Tetradecanoylphorbol Acetate/toxicity
EDAT- 1991/09/01 00:00
MHDA- 1991/09/01 00:01
CRDT- 1991/09/01 00:00
PHST- 1991/09/01 00:00 [pubmed]
PHST- 1991/09/01 00:01 [medline]
PHST- 1991/09/01 00:00 [entrez]
AID - 10.1093/carcin/12.9.1619 [doi]
PST - ppublish
SO  - Carcinogenesis. 1991 Sep;12(9):1619-25. doi: 10.1093/carcin/12.9.1619.