PMID- 19100885
OWN - NLM
STAT- MEDLINE
DCOM- 20090128
LR  - 20131121
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 632
IP  - 1
DP  - 2009 Jan 19
TI  - Dispersive liquid-liquid microextraction followed by high-performance liquid 
      chromatography as an efficient and sensitive technique for simultaneous 
      determination of chloramphenicol and thiamphenicol in honey.
PG  - 80-5
LID - 10.1016/j.aca.2008.10.068 [doi]
AB  - Dispersive liquid-liquid microextraction (DLLME) coupled with high-performance 
      liquid chromatography-variable wavelength detector (HPLC-VWD) was developed for 
      extraction and determination of chloramphenicol (CAP) and thiamphenicol (THA) in 
      honey. In this extraction method, 1.0 mL of acetonitrile (as dispersive solvent) 
      containing 30 microL 1,1,2,2-tetrachloroethane (as extraction solution) was 
      rapidly injected by syringe into a 5.00-mL water sample containing the analytes, 
      thereby forming a cloudy solution. After extraction, phase separation was 
      performed by centrifugation and the enriched analytes in the sedimented phase 
      were determined by HPLC-VWD. Some important parameters, such as the nature and 
      volume of extraction solvent and dispersive solvent, extraction time, sample 
      solution pH, sample volume and salt effect were investigated and optimized. Under 
      the optimum extraction condition, the method yields a linear calibration curve in 
      the concentration range from 3 to 2000 microg kg(-1) for target analytes. The 
      enrichment factors for CAP and THA were 68.2 and 87.9, and the limits of 
      detection (S/N=3) were 0.6 and 0.1 microg kg(-1), respectively. The relative 
      standard deviations (RSDs) for the extraction of 10 microg kg(-1) of CAP and THA 
      were 4.3% and 6.2% (n=6). The main advantages of DLLME-HPLC method are simplicity 
      of operation, rapidity, low cost, high enrichment factor, high recovery, good 
      repeatability and extraction solvent volume at microliter level. Honey samples 
      were successfully analyzed using the proposed method.
FAU - Chen, Huaixia
AU  - Chen H
AD  - Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic 
      Functional Molecules & College of Chemistry and Chemical Engineering, Hubei 
      University, Wuhan 430062, PR China. hxch@hubu.edu.cn
FAU - Chen, Hui
AU  - Chen H
FAU - Ying, Jun
AU  - Ying J
FAU - Huang, Jianlin
AU  - Huang J
FAU - Liao, Lei
AU  - Liao L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081105
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Solvents)
RN  - 66974FR9Q1 (Chloramphenicol)
RN  - FLQ7571NPM (Thiamphenicol)
SB  - IM
MH  - Chloramphenicol/*analysis/*chemistry
MH  - Chromatography, High Pressure Liquid/*instrumentation/*methods
MH  - Honey/*analysis
MH  - Hydrogen-Ion Concentration
MH  - Molecular Structure
MH  - Osmolar Concentration
MH  - Sensitivity and Specificity
MH  - Solvents
MH  - Thiamphenicol/*analysis/*chemistry
MH  - Time Factors
EDAT- 2008/12/23 09:00
MHDA- 2009/01/29 09:00
CRDT- 2008/12/23 09:00
PHST- 2008/09/20 00:00 [received]
PHST- 2008/10/25 00:00 [revised]
PHST- 2008/10/28 00:00 [accepted]
PHST- 2008/12/23 09:00 [entrez]
PHST- 2008/12/23 09:00 [pubmed]
PHST- 2009/01/29 09:00 [medline]
AID - S0003-2670(08)01872-2 [pii]
AID - 10.1016/j.aca.2008.10.068 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2009 Jan 19;632(1):80-5. doi: 10.1016/j.aca.2008.10.068. Epub 
      2008 Nov 5.