PMID- 19101507
OWN - NLM
STAT- MEDLINE
DCOM- 20090213
LR  - 20101118
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 379
IP  - 2
DP  - 2009 Feb 6
TI  - RhoA induces expression of inflammatory cytokine in adipocytes.
PG  - 288-92
LID - 10.1016/j.bbrc.2008.12.040 [doi]
AB  - Rho GTPase regulates actin cytoskeleton organization and assembly in many cell 
      types, however, its significance in adipose tissue is not well characterized. 
      Here, we demonstrate high RhoA activity in adipose tissues of C57BL/6J mice. To 
      determine the effect of RhoA activation on 3T3-L1 cells, stable cell lines 
      overexpressing G14VRhoA fused to destabilizing domain of FKBP12 (DD-G14VRhoA-L1) 
      were generated. Treatment of DD-G14VRhoA-L1 cells with Shield1 following their 
      differentiation into adipocytes, resulted in the appearance of thick cortical 
      actin filaments, and increased the mRNA expression levels of plasminogen 
      activator inhibitor type-1 (PAI-1) and monocyte chemoattractant protein-1 
      (MCP-1). The induction of PAI-1 and MCP-1 was inhibited by treatment with a 
      Rho-associated kinase (ROCK) inhibitor, Y-27632. In 3T3-L1 adipocytes, tumor 
      necrosis factor-alpha activated RhoA and increased mRNA expression of PAI-1 and 
      MCP-1, and their treatment with Y-27632 partially inhibited these changes. The 
      results indicate that RhoA-ROCK pathway induces inflammatory cytokine expression 
      in adipocytes.
FAU - Nakayama, Yuki
AU  - Nakayama Y
AD  - Department of Organismal Biosystems, Graduate School of Frontier Biosciences, 
      Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
FAU - Komuro, Ryutaro
AU  - Komuro R
FAU - Yamamoto, Akiko
AU  - Yamamoto A
FAU - Miyata, Yugo
AU  - Miyata Y
FAU - Tanaka, Masaki
AU  - Tanaka M
FAU - Matsuda, Morihiro
AU  - Matsuda M
FAU - Fukuhara, Atsunori
AU  - Fukuhara A
FAU - Shimomura, Iichiro
AU  - Shimomura I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081225
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Amides)
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (Pyridines)
RN  - 0 (Serpin E2)
RN  - 0 (Serpine2 protein, mouse)
RN  - 0 (Serpins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 138381-45-0 (Y 27632)
RN  - EC 2.7.11.1 (rho-Associated Kinases)
RN  - EC 3.6.5.2 (rhoA GTP-Binding Protein)
SB  - IM
MH  - 3T3 Cells
MH  - Adipocytes/*enzymology
MH  - Amides/pharmacology
MH  - Animals
MH  - Chemokine CCL2/biosynthesis
MH  - Cytokines/*biosynthesis
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Protein Kinase Inhibitors/pharmacology
MH  - Pyridines/pharmacology
MH  - Serpin E2
MH  - Serpins/biosynthesis
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - rho-Associated Kinases/antagonists & inhibitors/metabolism
MH  - rhoA GTP-Binding Protein/antagonists & inhibitors/*metabolism
EDAT- 2008/12/23 09:00
MHDA- 2009/02/14 09:00
CRDT- 2008/12/23 09:00
PHST- 2008/12/08 00:00 [received]
PHST- 2008/12/09 00:00 [accepted]
PHST- 2008/12/23 09:00 [entrez]
PHST- 2008/12/23 09:00 [pubmed]
PHST- 2009/02/14 09:00 [medline]
AID - S0006-291X(08)02426-1 [pii]
AID - 10.1016/j.bbrc.2008.12.040 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2009 Feb 6;379(2):288-92. doi: 
      10.1016/j.bbrc.2008.12.040. Epub 2008 Dec 25.