PMID- 19102689
OWN - NLM
STAT- MEDLINE
DCOM- 20090323
LR  - 20090121
IS  - 1520-4812 (Electronic)
IS  - 1043-1802 (Linking)
VI  - 20
IP  - 1
DP  - 2009 Jan
TI  - Conjugation of latent membrane protein (LMP)-2 epitope to gold nanoparticles as 
      highly immunogenic multiple antigenic peptides for induction of Epstein-Barr 
      virus-specific cytotoxic T-lymphocyte responses in vitro.
PG  - 24-31
LID - 10.1021/bc800167q [doi]
AB  - Nasopharyngeal carcinoma is a neoplasm with a high incidence in Southeast Asia, 
      and it is strongly associated with Epstein-Barr virus (EBV) activation involving 
      the expression of a weakly immunogenic protein, namely, latent membrane protein 
      (LMP)-2. Previous immunological studies already identified the human leukocyte 
      antigen (HLA)-A11 restricted peptide epitope (SSCSSCPLSK) in the LMP-2 antigen. 
      In this work, we prepared gold nanoparticle (AuNP)-peptide conjugate 1 by 
      treating the nanoparticles with the N-cysteinated LMP-2 epitope. The AuNP-peptide 
      conjugates have been characterized by TEM (15-24 nm in diameter) and UV-vis 
      spectroscopy (surface plasmon resonance absorption band at lambda(max) = 520 nm). 
      In the presence of a CALNN capping peptide, the AuNP-peptide conjugates are 
      stable in solution without aggregation at room temperature for at least 48 h. By 
      ELIspot studies, AuNP-peptide conjugate 1 was found to elicit a significantly 
      stronger INF-gamma response [number of spot forming cells (SPC) = 727 +/- 198] 
      from peripheral blood mononuclear cells of healthy HLA-A11 donors when compared 
      to that induced by the unconjugated LMP-2 peptides (SFC = 73 +/- 28). Further 
      studies showed that dendritic cells treated with conjugate 1 can effect CD8+ 
      T-cell activation leading to epitope-specific cytotoxic T lymphocyte killing 
      responses in vitro.
FAU - Cheung, Wai-Hung
AU  - Cheung WH
AD  - Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic 
      University, Hung Hom, Kowloon, Hong Kong.
FAU - Chan, Vera Sau-Fong
AU  - Chan VS
FAU - Pang, Heung-Wing
AU  - Pang HW
FAU - Wong, Man-Kin
AU  - Wong MK
FAU - Guo, Zhi-Hong
AU  - Guo ZH
FAU - Tam, Paul Kwong-Hang
AU  - Tam PK
FAU - Che, Chi-Ming
AU  - Che CM
FAU - Lin, Chen-Lung
AU  - Lin CL
FAU - Yu, Wing-Yiu
AU  - Yu WY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Bioconjug Chem
JT  - Bioconjugate chemistry
JID - 9010319
RN  - 0 (Antigens, Viral)
RN  - 0 (EBV-associated membrane antigen, Epstein-Barr virus)
RN  - 0 (Viral Matrix Proteins)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Antigens, Viral/*immunology
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Cytotoxicity, Immunologic
MH  - Gold
MH  - Herpesvirus 4, Human/*immunology
MH  - Humans
MH  - Lymphocyte Activation/immunology
MH  - Metal Nanoparticles/*chemistry
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - Viral Matrix Proteins/chemistry/*immunology
EDAT- 2008/12/24 09:00
MHDA- 2009/03/24 09:00
CRDT- 2008/12/24 09:00
PHST- 2008/12/24 09:00 [entrez]
PHST- 2008/12/24 09:00 [pubmed]
PHST- 2009/03/24 09:00 [medline]
AID - 10.1021/bc800167q [pii]
AID - 10.1021/bc800167q [doi]
PST - ppublish
SO  - Bioconjug Chem. 2009 Jan;20(1):24-31. doi: 10.1021/bc800167q.