PMID- 19103162
OWN - NLM
STAT- MEDLINE
DCOM- 20090206
LR  - 20090116
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 379
IP  - 1
DP  - 2009 Jan 30
TI  - Efficient osteoblast differentiation from mouse bone marrow stromal cells with 
      polylysin-modified adenovirus vectors.
PG  - 127-32
LID - 10.1016/j.bbrc.2008.12.055 [doi]
AB  - Bone marrow stromal cells (BMSCs) are expected to be a source for tissue 
      regeneration because they can differentiate into multiple cell types. 
      Establishment of efficient gene transfer systems for BMSCs is essential for their 
      application to regenerative medicine. In this study, we compared the transduction 
      efficiency in mouse primary BMSCs by using fiber-modified adenovirus (Ad) 
      vectors, and demonstrated that AdK7, which harbors a polylysin (K7) peptide in 
      the C-terminus of the fiber knob, could efficiently express a transgene in BMSCs. 
      Notably, AdK7 robustly drove transgene expression in more than 90% of the BMSCs 
      at 3,000 vector particles/cell. Furthermore, we showed that in vitro and in vivo 
      osteogenic potential of BMSCs was dramatically promoted by the transduction of 
      Runx2 gene using AdK7. These results indicate that this transduction system could 
      be a powerful tool for therapeutic applications based on BMSCs.
FAU - Tashiro, Katsuhisa
AU  - Tashiro K
AD  - Laboratory of Gene Transfer and Regulation, National Institute of Biomedical 
      Innovation, Ibaraki, Osaka, Japan.
FAU - Kondo, Asami
AU  - Kondo A
FAU - Kawabata, Kenji
AU  - Kawabata K
FAU - Sakurai, Haruna
AU  - Sakurai H
FAU - Sakurai, Fuminori
AU  - Sakurai F
FAU - Yamanishi, Koichi
AU  - Yamanishi K
FAU - Hayakawa, Takao
AU  - Hayakawa T
FAU - Mizuguchi, Hiroyuki
AU  - Mizuguchi H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081225
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Core Binding Factor Alpha 1 Subunit)
RN  - 0 (Runx2 protein, mouse)
RN  - 25104-18-1 (Polylysine)
SB  - IM
MH  - Adenoviridae/genetics
MH  - Animals
MH  - Bone Marrow Cells/*cytology
MH  - *Cell Culture Techniques
MH  - *Cell Differentiation
MH  - Core Binding Factor Alpha 1 Subunit/genetics
MH  - Genetic Vectors/chemistry/genetics
MH  - Mice
MH  - Osteoblasts/cytology/*physiology
MH  - Osteogenesis
MH  - Polylysine/chemistry
MH  - Stromal Cells/cytology
MH  - Transduction, Genetic/*methods
EDAT- 2008/12/24 09:00
MHDA- 2009/02/07 09:00
CRDT- 2008/12/24 09:00
PHST- 2008/12/02 00:00 [received]
PHST- 2008/12/07 00:00 [accepted]
PHST- 2008/12/24 09:00 [entrez]
PHST- 2008/12/24 09:00 [pubmed]
PHST- 2009/02/07 09:00 [medline]
AID - S0006-291X(08)02398-X [pii]
AID - 10.1016/j.bbrc.2008.12.055 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2009 Jan 30;379(1):127-32. doi: 
      10.1016/j.bbrc.2008.12.055. Epub 2008 Dec 25.