PMID- 19103255
OWN - NLM
STAT- MEDLINE
DCOM- 20090413
LR  - 20151119
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 451
IP  - 1
DP  - 2009 Feb 13
TI  - Vomeronasal neurons promote synaptic formation on dendritic spines but not 
      dendritic shafts in primary culture of accessory olfactory bulb neurons.
PG  - 20-4
LID - 10.1016/j.neulet.2008.12.009 [doi]
AB  - To investigate the morphological changes of accessory olfactory bulb (AOB) 
      neurons arising from pheromonal signals, a coculture system of AOB neurons and 
      vomeronasal (VN) neurons had been established. Our previous study indicates that 
      under coculture condition, the density of dendritic spines of an AOB neuron is 
      less and the individual spine-head volume is larger than those under monoculture 
      condition. In this study, to determine whether these differences in the dendrites 
      of AOB neurons reflect the differences in synapse formation and synaptic 
      properties, we observed these cultured cells by electron microscopy. Various 
      synapses were observed under each culture condition. Synapses were classified on 
      the basis of their postsynaptic structure and the size of postsynaptic density 
      (PSD) was measured. Under the coculture condition with VN neurons, synapses on 
      dendritic spines, which formed between AOB neurons, were observed frequently. In 
      contrast, many synapses were formed on dendritic shafts under monoculture 
      condition. The PSD of asymmetrical synapses on the spines under coculture 
      condition was larger than that under monoculture condition. Moreover, some 
      dendrodendritic reciprocal synapses were found only in coculture. We confirmed 
      synapse formation between VN axons and AOB dendrites by immunohistochemical 
      electron microscopy; thus, the characteristics of synapses between AOB neurons 
      are considered to be modified by the synaptic contacts with VN axons.
FAU - Moriya-Ito, Keiko
AU  - Moriya-Ito K
AD  - Laboratory of Anatomy and Cell Biology, Department of Neuroscience Basic 
      Technology, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu, 
      Tokyo 183-8526, Japan.
FAU - Endoh, Kentaroh
AU  - Endoh K
FAU - Ichikawa, Masumi
AU  - Ichikawa M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081216
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Biomarkers)
RN  - 0 (Pheromones)
RN  - 0 (Synaptophysin)
SB  - IM
MH  - Animals
MH  - Biomarkers/analysis/metabolism
MH  - Cells, Cultured
MH  - Dendritic Spines/physiology/*ultrastructure
MH  - Microscopy, Immunoelectron
MH  - Olfactory Bulb/physiology/*ultrastructure
MH  - Olfactory Pathways/physiology/*ultrastructure
MH  - Olfactory Receptor Neurons/physiology/*ultrastructure
MH  - Pheromones/metabolism
MH  - Presynaptic Terminals/physiology/ultrastructure
MH  - Rats
MH  - Rats, Wistar
MH  - Smell/physiology
MH  - Synapses/physiology/*ultrastructure
MH  - Synaptic Transmission/drug effects/physiology
MH  - Synaptophysin/analysis/metabolism
MH  - Vomeronasal Organ/physiology/*ultrastructure
EDAT- 2008/12/24 09:00
MHDA- 2009/04/14 09:00
CRDT- 2008/12/24 09:00
PHST- 2008/11/06 00:00 [received]
PHST- 2008/12/05 00:00 [revised]
PHST- 2008/12/06 00:00 [accepted]
PHST- 2008/12/24 09:00 [entrez]
PHST- 2008/12/24 09:00 [pubmed]
PHST- 2009/04/14 09:00 [medline]
AID - S0304-3940(08)01712-6 [pii]
AID - 10.1016/j.neulet.2008.12.009 [doi]
PST - ppublish
SO  - Neurosci Lett. 2009 Feb 13;451(1):20-4. doi: 10.1016/j.neulet.2008.12.009. Epub 
      2008 Dec 16.