PMID- 19103925
OWN - NLM
STAT- MEDLINE
DCOM- 20090323
LR  - 20211020
IS  - 1098-5530 (Electronic)
IS  - 0021-9193 (Print)
IS  - 0021-9193 (Linking)
VI  - 191
IP  - 5
DP  - 2009 Mar
TI  - Retentive memory of bacteria: Long-term regulation of dehalorespiration in 
      Sulfurospirillum multivorans.
PG  - 1650-5
LID - 10.1128/JB.00597-08 [doi]
AB  - The gram-negative, strictly anaerobic epsilonproteobacterium Sulfurospirillum 
      multivorans is able to gain energy from dehalorespiration with tetrachloroethene 
      (perchloroethylene [PCE]) as a terminal electron acceptor. The organism can also 
      utilize fumarate as an electron acceptor. Prolonged subcultivation of S. 
      multivorans in the absence of PCE with pyruvate as an electron donor and fumarate 
      as an electron acceptor resulted in a decrease of PCE dehalogenase (PceA) 
      activity. Concomitantly, the pceA transcript level equally decreased as shown by 
      reverse transcriptase PCR. After 35 subcultivations (approximately 105 
      generations), a pceA transcript was not detectable and the PceA protein and 
      activity were completely absent. In such long-term subcultivated S. multivorans 
      cells, the biosynthesis of catalytically active PceA was restored to the initial 
      level within about 50 h (approximately three generations) by the addition of PCE 
      or trichloroethene. Single colonies obtained from PceA-depleted cultures were 
      able to induce PCE dechlorination, indicating that long-term subcultured cells 
      still contained the functional pceA gene. The results point to a novel type of 
      long-term regulation of PCE dehalogenase gene expression in S. multivorans.
FAU - John, Markus
AU  - John M
AD  - Friedrich-Schiller University, Institute for Microbiology, Jena, Germany.
FAU - Rubick, Raffael
AU  - Rubick R
FAU - Schmitz, Roland P H
AU  - Schmitz RP
FAU - Rakoczy, Jana
AU  - Rakoczy J
FAU - Schubert, Torsten
AU  - Schubert T
FAU - Diekert, Gabriele
AU  - Diekert G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081219
PL  - United States
TA  - J Bacteriol
JT  - Journal of bacteriology
JID - 2985120R
RN  - 0 (Bacterial Proteins)
RN  - 0 (Culture Media)
RN  - 4R7X1O2820 (Chlorine)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.- (tetrachloroethene dehalogenase)
RN  - TJ904HH8SN (Tetrachloroethylene)
SB  - IM
MH  - Anaerobiosis
MH  - Bacterial Proteins/genetics/metabolism
MH  - Chlorine/*metabolism
MH  - Culture Media
MH  - Epsilonproteobacteria/*enzymology/genetics/*growth & development/metabolism
MH  - *Gene Expression Regulation, Bacterial
MH  - Oxidoreductases/genetics/*metabolism
MH  - Tetrachloroethylene/*metabolism
MH  - Time Factors
PMC - PMC2648215
EDAT- 2008/12/24 09:00
MHDA- 2009/03/24 09:00
PMCR- 2009/09/01
CRDT- 2008/12/24 09:00
PHST- 2008/12/24 09:00 [entrez]
PHST- 2008/12/24 09:00 [pubmed]
PHST- 2009/03/24 09:00 [medline]
PHST- 2009/09/01 00:00 [pmc-release]
AID - JB.00597-08 [pii]
AID - 0597-08 [pii]
AID - 10.1128/JB.00597-08 [doi]
PST - ppublish
SO  - J Bacteriol. 2009 Mar;191(5):1650-5. doi: 10.1128/JB.00597-08. Epub 2008 Dec 19.